Epidemiology and molecular characterization of Cystic echinococcosis in cattle and Cysticercosis in pigs
| dc.contributor.advisor | Upadhyay, A.K. | |
| dc.contributor.author | Pathak, Ayushi | |
| dc.date.accessioned | 2022-10-09T09:52:49Z | |
| dc.date.available | 2022-10-09T09:52:49Z | |
| dc.date.issued | 2022-06 | |
| dc.description.abstract | The present study was undertaken to study the epidemiology of Echinococcosis in cattle and Cysticercosis in pigs through slaughter house study of Uttarakhand. During this study, 264 buffaloes and 100 pigs were studied. The overall prevalence rate of Hydatidosis was 13.26% {Nainital (n=172)- 14.53%, Bageshwar (n=25), 12%, Almora (n=18)-11.11%, US Nagar (n=38), 7.89% and Rampur (n=11)- 18.18%}. For Cysticercosis, the overall prevalence rate was 3% {US Nagar (n=60)-1.66%, Nainital (n=30)-3.33% and Bageshwar (10)-10%}. Both the diseases were found to be independent of the geographical location of the animals. The average prevalence recorded in lungs, liver and both lungs & liver was 14.28%, 71.42% and 14.28%, respectively without significant statistical difference. Genderwise prevalence revealed no significant difference statistically and was higher in females in both the diseases (hydatidosis- 15.43% and cysticercosis-3.03%) and lower in males (hydatidosis-9.8% and cysticercosis-2.94%). Statistical significant difference could not be observed on age-wise prevalence considerations {Hydatidosis- 6-7y (16.66%), 4-5y (14.63%) and 2-3y (8%); cysticercosis-1-2y (4.16%) and 3-4 years (2.77%)}. Various types of cysts were recovered from different organs without statistical significant difference in rate of infection {single (lungs-80%, liver-80%) as well as multiple (lungs-20%, liver-20%)}. The overall fertility rates found in the cysts were 71.42% (lungs-40%, liver-80%, lungs & liver-60%). The overall viability rate of the cysts recovered from different organs was 80% (lungs-50%, liver- 80%, lungs & liver-100%). Significant difference was not observed in sizes of cyst and their viability {3-6cm (85.71%); >6cm (77.78%)}. DNA was extracted from 25 Echinococcus samples and 3 T. solium samples. Cox1 gene amplification fragment length observed was 440bp in Echinococcus and 420bp in Cysticercus. Purification of PCR products lead to clear products of 340 bp for US Nagar isolate; 338bp for Nainital isolate; 319 bp for Bageshwar isolate; 330 bp for Almora isolate; 339 bp for Rampur isolate for Echinococcus. For Cysticercus, clear products of 322 bp for US Nagar isolate; 418 bp for Bageshwar isolate and 354 bp for Nainital isolate were obtained. Phylogenetic analysis of the isolates show that Echinococcus isolates from the present study are 100% similar to the isolates from South Korea, Iran, Italy, Israel, Turkey and Iraq (G1 isolate). The T. solium isolates share 97.92% resemblance with Mexico isolate (TsChb4), 98.02% with Bangalore isolate, 98.59% with Meghalaya isolate (Ts-MI) and, 97.99% with Uttar Pradesh isolate (TsInd D). The Bageshwar isolate of T. solium is shown to be the root of the phylogenetic tree with other isolates being the branches. This is the first report of prevalence of G1 isolate of Echinococcus in buffaloes. Asian isolate of T. solium is found in the pigs of Uttarakhand, thus explaining the significance of this study in understanding the zoonotic potential of both the parasites. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810188707 | |
| dc.keywords | Cystic echinococcosis, cattle, Cysticercosis, pigs | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 108 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145 | en_US |
| dc.research.problem | Cattle | en_US |
| dc.sub | Veterinary Public Health | en_US |
| dc.theme | Cystic echinococcosis | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | Epidemiology and molecular characterization of Cystic echinococcosis in cattle and Cysticercosis in pigs | en_US |
| dc.type | Thesis | en_US |