Molecular Dissection of AGPase to Improve Wheat Productivity
| dc.contributor.advisor | Sikka, V.K. | |
| dc.contributor.author | Batra, Ritu | |
| dc.date.accessioned | 2017-06-03T04:18:53Z | |
| dc.date.available | 2017-06-03T04:18:53Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | The present investigation was conducted towards understanding role of ADP-glucose pyrophosphorylase (AGPase) in developing grains’ starch biosynthesis among diverse wheat lines including disomic chromosome substitution (DCS) lines of C-591 in the background of Chinese Spring, dwarf wheat varieties. isogenic lines and for WH-711 X WH 542. The experiment was laid out in completely randomized block design with three replications. The traits like grain growth rate (GGR) and AGPase enzyme activity were also measured for developing grains at 10th, 20th and 30th days after anthesis (DAA). Metabolites like total sugars, reducing sugars, non-reducing sugars, total starch, amylose and amylopectin were also quantified using wheat flour of the test lines. The recorded data was subjected to appropriate statistical analysis to arrive at inferences on the experiments in the study. Critical analysis of the data led us to conclude that determinants for AGPase expression are present across the genome over multiple chromosomes which are active at particular grain filling stages like during first half, chromosome 1B and 2D played major role whereas second half was mainly controlled by chromosome 6A and 5B. Hybridization efforts have improved grain attributes and yield components of WH-542 with ~28.6 % and 42 % enhancement in grain wt. per spike and yield per plant respectively. Maximum amylose (%) was observed in DCS line 2D which can be used to produce functional wheat flour. Grain yield was found to be significantly associated with grains/spike and plant biomass in case of DCS lines. Partitioning the variation by path analysis indicates grains per spike, though had less direct effect but its impact on grain yield through indirect effect via tiller no./plant and AGPase activity at 20th DAA was high suggesting their importance in yield improvement efforts. AGPase activity analysis suggests major contribution of chromosome 2D towards yield in a specific temperature window of 22±1.5oC during initial stages of grain filling which can be used as a screening tool for terminal heat tolerant genotypes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810014817 | |
| dc.language.iso | en | en_US |
| dc.publisher | CCSHAU | en_US |
| dc.sub | Molecular Biology and Biotechnology | |
| dc.subject | grain, wheats, starch, planting, developmental stages, yields, enzymes, genes, chromosomes, biological development | en_US |
| dc.these.type | Ph.D | |
| dc.title | Molecular Dissection of AGPase to Improve Wheat Productivity | en_US |
| dc.type | Thesis | en_US |
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