Antioxidant profiling in ten selected wild leafy vegetables of Kumaun region
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Date
2017-07
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In recent times, considerable increase in concern regarding the harmful effect of oxidants on human health, a great deal of attention is being given to the exploration of such sources that might play a major role in neutralizing these damaging species. Antioxidants have acquired an excellent position in this regard as they known to provide protection against the free radicals. Therefore, natural antioxidants are considered comparatively safer and healthier than synthetic ones. To ascertain the potential of wild leafy vegetables as an important source of natural antioxidants, the antioxidant activity in ten different wild leafy vegetables were collected from Kumaun region of Uttarakhand in methanolic extract and evaluated by using three different assays namely, 2, 2-diphenyl-1- picrylhydrazyl (DPPH), ferrous chelating ability (FCA), total antioxidant activity (TAA) . In addition, total phenolic content (TPC) and total flavonoid content (TFC) are also measured by using Folin Ciocalteu reagent and flavonoid assay. In the present study, all ten wild leafy vegetables exhibited the presence of phytochemical components i.e., carbohydrates, protein, fat, terpene and flavonoids. The mean values (mean of five conc.) ranged from 91.11 (I.cairica ) to 41.74 (S.americanum) in DPPH (%), 93.98 (I.cairica) to 46.62 (S. americanum) in FCA (%); 9.04 (I. cairica) to 2.9 (S. americanum) in TAA (µg AAE/mg extract); 9.52 ± 1.0 (O. debilis) to 2.5 ± 2.0 (S. americanum) in TPC (µg GAE/mg extract) and7.8 ± 0.12 (I. cairica) to 1.9 ± 0.16 (S. americanum) in TFC (µg QE/mg extract). Among species, comparatively higher values in DPPH, FCA, TAA and TFC are in I. cairica whereas TPC is in O. debilis. Thus, the species I. cairica and O. debilis have high amount of antioxidants thus, they are recommended for consumption. Significant and positive correlation was found between all possible combinations with a highly significant between DPPH vs. FCA, DPPH vs.TAA, TAA vs. FCA and TPC vs. TFC except between TPC vs. TAA, where it was negatively correlated. Thus, the results of the present study, supports the view that all selected wild leafy vegetables are promising source of natural antioxidants.
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