Investigation on phytochemical attributes and antioxidative potential of Pleurotus sajor-caju (Fr.) Singer
dc.contributor.advisor | Punetha, Himanshu | |
dc.contributor.author | Srinivas, Arji Ravi Chandra | |
dc.date.accessioned | 2022-11-13T06:45:09Z | |
dc.date.available | 2022-11-13T06:45:09Z | |
dc.date.issued | 2022-09 | |
dc.description.abstract | The current work details about the evaluation of the phytochemical composition of different polarity extracts and the biological activities of mushroom P.sajor-caju sample acquired from mushroom research and training centre, G. B. Pant University of Agriculture & Technology, Pantnagar. Twenty one compounds were identified with the help of GC-MS representing 98.61% of total methanolic extract composition. Cis-Vaccenic acid, Beta-Monolinolein, ergosterol derivatives are major compounds identified. Fifty two compounds were identified with the help of GC-MS representing 99.48 % of total petroleum ether extract composition. Linoleic acid, Eicosane, Heneicosane and Hexatriacontane were the major compounds identified. The Methanolic extract showed potent antioxidant activity in terms of DPPH radical scavenging, metal chelating activity and reducing power activity having IC50 value of 677.73±1.72μg/mL, 4.25±0.05 mg/mL and 9.66±0.11 mg/mL respectively in comparison to petroleum ether extract which might be due to presence of Cis-Vaccenic acid, Beta-monolinolein. Total phenolic content (TPC), Total flavonoid content (TFC) and Total antioxidant content (TAC) of methanol and petroleum ether extract were also evaluated. TPC, TFC and TAC were higher in methanol extract having 21.3±0.006 mg/gm, 3.43±0.002 mg/gm, and 19.8±0.65 mg/gm, respectively. Crude protein content was observed as 19.78% along with mineral composition Fe, Mn, Cu and Zn as 0.0156±0.89 mg/g, 0.029±1.56 mg/g, 0.018±1.25 mg/g and 0.085±1.23 mg/g respectively. The molecular docking of bioactive compounds Ergosterol and β-glucans of P sajor-caju against Covid-19 spike proteins 6vsb, 6lxt, 6lvn and 6vw1 was performed using PyRx software by autodock wizard as the engine for docking. The results obtained shows binding energies of 6vsb, 6lxt, 6lvn and 6vw1 with ergosterol as -9.3 kcal/mol, -7.9 kcal/mol, -7.1 kcal/mol and -8.7 kcal/mol respectively. The binding energies of 6vsb, 6lxt, 6lvn and 6vw1 with β-glucans as -6.8 kcal/mol, -7.5 kcal/mol, -6.2 kcal/mol and -7.5 kcal/mol respectively. The mushroom P. sajor-caju thus have potent medicinal properties and if explored further might be potent antioxidant and antiviral cost-effective agent compared to synthetically derived agents from pharma industries. | en_US |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810189842 | |
dc.language.iso | English | en_US |
dc.pages | 70 | en_US |
dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145 | en_US |
dc.research.problem | Pleurotus sajor-caju | en_US |
dc.sub | Biochemistry | en_US |
dc.theme | Mushrooms | en_US |
dc.these.type | M.Sc | en_US |
dc.title | Investigation on phytochemical attributes and antioxidative potential of Pleurotus sajor-caju (Fr.) Singer | en_US |
dc.type | Thesis | en_US |
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