BIOCONTROL POTENTIAL OF QUORUM QUENCHING RHIZOBACTERIA AGAINST SOFT ROT PATHOGEN, Pectobacterium carotovorum subsp. carotovorum.
| dc.contributor.advisor | Tamilvendan, K. | |
| dc.contributor.author | SARANYA PACKIALAKSHMI, J. | |
| dc.date.accessioned | 2023-01-04T09:28:45Z | |
| dc.date.available | 2023-01-04T09:28:45Z | |
| dc.date.issued | 2022-02-15 | |
| dc.description.abstract | Pectobacterium carotovorum subsp. carotovorum (Pcc) is a brute force pathogen causing necrotic soft rot, virulence of which is induced by quorum sensing regulated production of plant cell wall degrading enzymes. Quorum quenching (QQ) by the disruption of the quorum sensing signals, the AHL molecules, is recently being proposed as the strategy for the biocontrol of plant pathogens. Employing QQ based suppression of plant pathogens with rhizobacteria, therefore, assumes a greater significance in the wake of development of multidrug resistance in plant pathogens. To harness the potential of QQ rhizobacteria in the biocontrol programme, ninety-six bacteria isolated from the rhizosphere of crops cultivated in farms of GKVK were screened for their QQ ability using the bioindicator Chromobacterium violaceum RU9. The in vitro screening yielded twenty-four isolates with variable QQ efficiency. Out of 24 isolates, the isolate BMR17 recorded significantly higher QQ efficiency of 93% while BRR05, BBR58, BBR60, BAR70, BAR75, BAR78, BAR79 and BFR83 exhibited efficiency between 86% and 92%. In vitro biocontrol assay of green gram seeds and in - vitro maceration attenuation assay on Potato, Carrot, Radish and Cucumber evidenced that BRR05, BMR17, BMR22, RAR39, BBR57, BAR79 and BFR86 showed efficient QQ biocontrol potential. The isolates BRR05, BMR17 and BFR86 degraded different long chain and short chain AHL molecules as revealed by HPLC chromatogram and heat map. Principal Component Analysis of the plant growth and yield parameters of Green gram as influenced by the inoculation of these three biocontrol isolates under greenhouse condition revealed that BFR86 (Priestia megaterium) is the potent QQ biocontrol agent against the pathogen Pcc followed by BMR17 (Staphylococcus hominis) and BBR57 (Priestia aryabhattai). | en_US |
| dc.identifier.other | Th-13145 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810191156 | |
| dc.keywords | QUORUM, SOFT ROT PATHOGEN | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 226 | en_US |
| dc.publisher | University of Agricultural Sciences, Bangalore | en_US |
| dc.sub | Agricultural Microbiology | en_US |
| dc.theme | QUORUM QUENCHING RHIZOBACTERIA | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | BIOCONTROL POTENTIAL OF QUORUM QUENCHING RHIZOBACTERIA AGAINST SOFT ROT PATHOGEN, Pectobacterium carotovorum subsp. carotovorum. | en_US |
| dc.type | Thesis | en_US |