STUDIES ON PHYSIOLOGY AND MANAGEMENT OF Fusarium oxysporum f.sp. lentis CAUSING WILT OF LENTIL”
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Date
2023-08-19
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BANDA UNIVERSITY OF AGRICULTURE & TECHNOLOGY, BANDA-210001, UTTAR PRADESH, INDIA
Abstract
Lentil (Lens esculenta) is one of the most important pulse crops of Bundelkhand
region of Uttar Pradesh. Wilt disease caused by Fusarium oxysporum f. sp. lentis is one of
the major constraints in production of lentil in this region which causes up to 50 % yield
loss. To minimize the yield loss caused by this disease the studies were conducted on the
topic of “Studies on Physiology and Management of Fusarium oxysporum f.sp. lentis
causing wilt of lentil” Management of Fusarium wilt of lentil is not possible by a single
method of plant disease management. Integrated disease management (integration of
bioagents and fungicides) is gaining much importance. Therefore, keeping in view, the above
facts following experiments were conducted. Laboratory experiments were conducted to
evaluate the effect of different pH (4.5-9.0) and temperature (5-350C) on colony growth of
pathogen and effect of media on colony growth and sporulation of pathogen. The pH (6.0)
and temperature (300C) were found best for mycelial growth of pathogen 75.86mm and
90mm respectively. The lentil seed extract Agar Medium found best for mycelial growth
(80mm) and V8 juice medium found best for sporulation (22.9x105
spores/ml). Indigenous
fungal and bacterial bio-agents were tested in vitro for their efficacy against pathogen.
Among six native fungal antagonists, T. harzianum (TH-2) was found highly effective
against the pathogen, which inhibited maximum mycelia growth (69.79%) of the pathogen
followed by T. harzianum (TH-5) which caused 65.62% inhibition in mycelia growth of
pathogen. Among bacterial antagonists P. fluorescens showed maximum (54.37%)
inhibition in mycelial growth of pathogen followed by B. subtilis (46.04%). The
compatibility of potential bio-agents was also tested through dual culture technique in which
P. fluorescens was found compatible to T. harzianum (TH-2) and Trichoderma asperelloides
(TA-1) without any inhibition of mycelial growth. Another experiment was conducted to
select the fungicide which was effective against pathogen and compatible with bioagents.
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Citation
. POOJA YADAV, {2023}, STUDIES ON PHYSIOLOGY AND MANAGEMENT OF Fusarium oxysporum f.sp. lentis CAUSING WILT OF LENTIL”, M.Sc. (Ag.), BANDA UNIVERSITY OF AGRICULTURE & TECHNOLOGY, BANDA-210001, UTTAR PRADESH, INDIA