MORPHOLOGICAL AND SSR MARKERS CHARECTERIZATION OF MANGO CLONES OF ALPHONSO
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Date
2010-07-16
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University of Agricultural Sciences GKVK, Bangalore
Abstract
In India, Alphonso is a well known, premier cultivar which
occupies the prime position. In this cultivar few clones with distinct
characters have been observed. The origin of all these clones is not
known but these are indigenous to different parts of India. It is highly
probable that the variation between the clones is due to eco-geographical
conditions they have been exposed to and micro or macro level mutation
from the parent cultivar Alphonso. The present study was conducted to
ascertain differences among eight mango clones of Alphonso by using
morphological and SSR marker approach during the year 2009 and
2010.
Generally, a wide variation was noticed in Alphonso clones with
respect to physical and chemical parameters of the fruit and percentage
incidence and extent of spongy tissue disorder. The dendrogram based
on 16 morphological marker data successfully distinguished all eight
Alphonso clones from one another. Based on this analysis, Alphonso
clones have been grouped in to two major clusters as ‘A’ and ‘B’. Cluster
‘A’ consisted of clones which are mainly collected from Bengaluru region
and the clones collected from Dharwad District were placed in cluster ‘B’.
The eight Alphonso clones and a Mallika variety were characterized
at genomic level using 16 SSR primers. Among them only 2 primers were
successful in establishing the differences between Alphonso clones and
Mallika variety but failed to distinguish between the Alphonso clones. A
dendrogram constructed by Ward’s method of clustering grouped all the
Alphonso clones separately from Mallika variety.
It can be concluded that, though the various Alphonso clones are
morphologically different from each other but it could not be ascertained
at genomic level by the presently employed set of SSR primers.
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