STUDIES ON GENETIC DIVERSITY OF PHOSPHORUS SOLUBILIZING RHIZOBIA OF RAJMASH (PHASEOLUS VULGARIS L.)

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dc.contributor.advisorKAUSHAL, RAJESH
dc.contributor.authorGAUTAM, SWATI
dc.date.accessioned2019-11-22T09:43:35Z
dc.date.available2019-11-22T09:43:35Z
dc.date.issued2019-11
dc.description.abstractABSTRACT The present investigations were conducted to determine the genetic diversity of P-solubilizing rhizobia of rajmash by amplified ribosomal DNA restriction analysis (ARDRA). A total of 50 isolates associated with rajmash were authenticated as Rhizobium spp. on the basis of different authentication tests,viz.congo red test, bromothymol blue test, growth in Hofer’s alkaline broth, ketolactose medium and plant infection test. Out of 50 rhizobial isolates, all were P-solubilizers, sixteen were siderophore producers, nine were HCN producers, eight isolates showed antagonism against Rhizoctonia solani Kuhn and five against Colletotrichum lindemuthianum Sacc. and Mangus. The maximum P-solubilization (295.74 µg/ml) was recorded for isolate KS and maximum IAA production (83.19 µg/ml) was noted for isolate Har4. Maximum siderophoreproduction with zone size of 19mm was recorded with the isolate Shl7 on CAS medium. On the basis of banding pattern generated through ARDRA analysis using restriction enzymes Alu I and Msp I, 19 isolates from agro-climatic zone-II were grouped into seven major phylotypes at 49 per cent similarity level, 15 isolates from agroclimatic zone-III were segregated into five major phylotypes with 49 per cent similarity and 16 isolates from agro-climatic zone-IV were grouped into seven major phylotypes at 57 per cent similarity level. Seed bacterization with 10 selected rhizobia resulted in significant increase in shoot height, root length, plant biomass and number of nodules over uninoculated control. Among 10 sequenced rhizobia, 4 isolates viz. Hab3, BhrB, Tis2 and San1 were identified as Rhizobium nepotum, 3 isolates viz. KAF, Har1 and Kal3 as Rhizobium indigoferae, isolate KS as Rhizobium etli, isolate Har2 as Rhizobium acidisoli and isolate Shl3 as Rhizobium pusense. Seed bacterization with R. etli strain KS+70% P showed considerable effect on increase in shoot height (86 cm), root length (28.30 cm), number of nodules (55.07 no./plant) and available NPK over uninoculated control at flowering stage as well as harvest of the crop.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810135999
dc.keywordsgenetic diversity,rajmash, amplified ribosomal , analysisen_US
dc.language.isoenen_US
dc.pages126+xen_US
dc.publisherUHF,NAUNIen_US
dc.subBasic Sciencesen_US
dc.subjectnullen_US
dc.themegenetic diversity,rajmash, amplified ribosomal , analysisen_US
dc.these.typePh.Den_US
dc.titleSTUDIES ON GENETIC DIVERSITY OF PHOSPHORUS SOLUBILIZING RHIZOBIA OF RAJMASH (PHASEOLUS VULGARIS L.)en_US
dc.typeThesisen_US
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