MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF WILD AND CULTIVATED Vigna SPECIES FOR DOMESTICATION RELATED TRAITS
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Date
2023-08-08
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BANDA UNIVERSITY OF AGRICULTURE &TECHNOLOGYBANDA-210001, UTTAR PRADESH (INDIA)
Abstract
Twenty-nine elite genotypes of mungbean were characterized morphologicallybyusing PPV&FRA DUS descriptors and deliberated for genetic variability parameters toknowthe variability in the studied material. Further, the genotypes were delineated usingmolecularmarkers (SCoT, SSR and gene based markers) to know diversity within and betweengenotypes.The DUS descriptors, plant habit and colour of premature pod did not showed anyvariationamong genotypes. The genotypes were distinct remarkably for the descriptors liketypeofgermination, anthocyanin pigmentation, time of flowering, plant growth habit, stemcolour,stem pubescence, leaflet lobes, leaf shape, colour and size, leaf vein colour, petiolecolour,flower colour, mature pod colour, pod pubescence, pod position, curvature of mature pod, podlength, seed colour, lustre and shape among the genotypes indicating their utilizationincharacterization, registration, protection and purity maintenance. In the present investigation,PCV was more than the GCV for all the fourteen quantitative traits showing the presenceofenvironmental influence on character expression. Further, the genetic variability was moreforthe characters viz., days to flower initiation, days to maturity, plant height, total number of podsper plant, pod length, number of seeds per pod, chlorophyll content index at 15 days, seedindexand seed yield per plant. High heritability and high genetic advance for the traits flowerinitiation, days to maturity, plant height, total number of pods per plant, pod length, numberofseeds per pod, chlorophyll content index at 15 days, chlorophyll content index at 30days,chlorophyll content index at 45 days and chlorophyll content index at 60 days, seedindexandseed yield per plant indicated the additive gene effects role and exploitation of simple selectionName of the student: Pragya Jee ID. No. : 2031
Semester : IV Programme : M.Sc. (Ag.)
Year of admission : 2021 Department : Genetics andPlantBreeding
Major Subject : Genetics and Plant
Breeding
Minor Subject : Plant Biotechnology
for these traits improvement. All the twenty-nine genotypes were characterized usingStartCodon Targeted (SCoT), Sequenced Characterised Amplified Region (SCAR), gene basedandSimple Sequence Repeats (SSR) markers for screening of these genotypes. Ten SCoT, oneSCAR, three gene based and seven SSR primers were used for the molecular characterizationwhile, a total of 96 alleles were detected by 10 SCoT markers in a size range of 100-1450bpwith maximum 14 fragments intensified by SCoT-5 followed by SCoT-11 with 11fragmentsand a total of 29 alleles were detected by 7 SSR, 1 SCAR and 3 gene based markers inarangeof 100-1100bp with maximum 05 fragments intensified by g779 followed by CEDG293andCEDG 211 with 4 fragments. The study has identified some informative primers that could be employed tocarryoutfuture valuable molecular assessments for the purpose of improvement in Vigna genotypes.
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PRAGYA JEE {2023}, MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF WILD AND CULTIVATED Vigna SPECIES FOR DOMESTICATION RELATED TRAITS, M.Sc. (Ag.), BANDA UNIVERSITY OF AGRICULTURE &TECHNOLOGYBANDA-210001, UTTAR PRADESH (INDIA)