EVALUATION OF CERTAIN LEPTOSPIRAL DIAGNOSTIC TESTS
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Date
1987
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Abstract
Dark-field microscopy and immunoperoxidase staining test for
the demonstration of antigen and microscopic agglutinatian (MAT)
and enzyme linked immunosorbant assay (ELISA) for the demonstration
of antibody were compared in experimentally infected calves with
scrovars Pomona and autumnalis. The darkfield microscopy was
found better than the immunopcroxidase staining both in blood and
urine samples Second centrifugation at l0000G always gave higher
positive results. {The MAT detected early serum antibodies than the
ELISA The late antibody detection by ELISA may probably be due
to the use of IgG fraction in the conjugate The ELISA exhibited
higher level of titres than MAT.
Description
TNV_CRN_1987_16(5)214-219
Keywords
Veterinary Science