Hybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybrids

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dc.contributor.advisorNavjyot Kaur
dc.contributor.authorDamanpreet Kaur
dc.date.accessioned2022-07-30T13:10:46Z
dc.date.available2022-07-30T13:10:46Z
dc.date.issued2021
dc.description.abstractThe present investigation entitled „Hybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybrids‟ was conducted at Seed Physiology Laboratory, Office of Director (Seeds), during 2019-2021. The seeds of maize hybrid PMH1, its female parent (LM13), male parent (LM14); and hybrid PMH 10, its female parent (LM23), male parent (LM24) were procured from Maize Section, Department of Plant Breeding and Genetics, PAU, Ludhiana. DUS (distinctness, uniformity and stability) were recorded by sowing the hybrids PMH 1, PMH 10 and their parents under field conditions and characters were recorded. Eight and seven DUS characters were found to be polymorphic which could distinguish hybrid PMH 1 and PMH 10, respectively, from their parental lines. SVI II of LM 14 was significantly higher than LM 13 and PMH 1. SVI I and SVI II of hybrid PMH 10 and male parent were significantly higher than female parent. SDS-PAGE analysis of hybrid PMH 1 revealed one polymorphic polypeptide band corresponding to polypeptide of 45 kDa in female and 50 kDa in male which can be used for confirming the hybridity of maize hybrid PMH 1; while hybrid PMH 10 did not reveal any polymorphism. Superoxide dismutase and esterase zymography of hybrid PMH 1 revealed polymorphism which can be employed to test PMH 1 hybrid purity; while these two isozymes did not recorded any polymorphic isozymic bands in hybrid PMH 10. Peroxidase zymography of both hybrids PMH 1 and PMH 10 revealed polymorphism which can be employed for testing hybrid purity of both the hybrids. In molecular analysis, out of 200 primers screened, six SSR markers viz., bnlg1036, umc2170, umc2069, bnlg1297, umc1227 and bnlg1754 were found to be polymorphic which had potential to identify hybrid purity of PMH 1. Seven primers viz., umc1627, umc1786, umc1366, phi080, bnlg1297, bnlg1203 and bnlg1036 were polymorphic and capable of identifying PMH 10 hybrid purityen_US
dc.identifier.citationDamanpreet Kaur (2021). Hybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybrids (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810185928
dc.keywordsDUS, hybrid purity, isozyme, maize, polymorphic, SDS-PAGEen_US
dc.language.isoEnglishen_US
dc.pages99en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemHybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybridsen_US
dc.subBotanyen_US
dc.themeHybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybridsen_US
dc.these.typeM.Scen_US
dc.titleHybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybridsen_US
dc.typeThesisen_US
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