Hybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybrids
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Date
2021
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Punjab Agricultural University, Ludhiana
Abstract
The present investigation entitled „Hybrid purity testing using morphological, physiological,
biochemical and molecular markers in maize (Zea mays L.) hybrids‟ was conducted at Seed
Physiology Laboratory, Office of Director (Seeds), during 2019-2021. The seeds of maize
hybrid PMH1, its female parent (LM13), male parent (LM14); and hybrid PMH 10, its female
parent (LM23), male parent (LM24) were procured from Maize Section, Department of Plant
Breeding and Genetics, PAU, Ludhiana. DUS (distinctness, uniformity and stability) were
recorded by sowing the hybrids PMH 1, PMH 10 and their parents under field conditions and
characters were recorded. Eight and seven DUS characters were found to be polymorphic
which could distinguish hybrid PMH 1 and PMH 10, respectively, from their parental lines.
SVI II of LM 14 was significantly higher than LM 13 and PMH 1. SVI I and SVI II of hybrid
PMH 10 and male parent were significantly higher than female parent. SDS-PAGE analysis
of hybrid PMH 1 revealed one polymorphic polypeptide band corresponding to polypeptide
of 45 kDa in female and 50 kDa in male which can be used for confirming the hybridity of
maize hybrid PMH 1; while hybrid PMH 10 did not reveal any polymorphism. Superoxide
dismutase and esterase zymography of hybrid PMH 1 revealed polymorphism which can be
employed to test PMH 1 hybrid purity; while these two isozymes did not recorded any
polymorphic isozymic bands in hybrid PMH 10. Peroxidase zymography of both hybrids
PMH 1 and PMH 10 revealed polymorphism which can be employed for testing hybrid purity
of both the hybrids. In molecular analysis, out of 200 primers screened, six SSR markers viz.,
bnlg1036, umc2170, umc2069, bnlg1297, umc1227 and bnlg1754 were found to be
polymorphic which had potential to identify hybrid purity of PMH 1. Seven primers viz.,
umc1627, umc1786, umc1366, phi080, bnlg1297, bnlg1203 and bnlg1036 were polymorphic
and capable of identifying PMH 10 hybrid purity
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Citation
Damanpreet Kaur (2021). Hybrid purity testing using morphological, physiological, biochemical and molecular markers in maize (Zea mays L.) hybrids (Unpublished M.Sc. thesis). Punjab Agricultural University, Ludhiana, Punjab, India.