Biosynthesis and characterization of silver nanoparticles using S. mukorossi extract possessing antimicrobial potential
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Date
2023-11-28
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College of Horticulture and Forestry Dr YSP UHF, Neri, Hamirpur(H.P.)
Abstract
The present study on the “Biosynthesis and characterization of silver nanoparticles using S.mukorossi extract possessing antimicrobial potential” were carried out in Department of Biotechnology, College of Horticulture and Forestry (Dr. Y.S. Parmar University of Horticulture and Forestry), Neri, Hamirpur (H.P) during 2022-2023. Sapindus mukorossi is an extremely valuable cultivated medicinal plant which is mostly found in hilly regions of India. In the lists of herbs, it is a popular herb in Ayurveda and is used as an important ingredient in cleansers and shampoos. It is well known for its folk medicinal values and known by several names like washnut, soapnut, soapberry, dodan and ritha. The use of this plant promoted considerable attention because of their various biological and pharmacological activities. The major compound isolated from genus Sapindus are saponins, triterpeniods, fatty acids and flavonoids are well known for their antimicrobial, antidiabetic, cytotoxic, fungicidal, and anti-inflammatory activities. The study was undertaken for biosynthesis and characterization of silver nanoparticles derived from S. mukorossi pericarp extract. The sample was collected from village Sheglagalu, Chail Chowk, Distt. Mandi (H.P). Synthesis was carried out using different solvents viz., methanol, chloroform and aqueous. It was found that distilled water acts as a better solvent for the synthesis of silver nanoparticles. The characterization of prepared silver nanoparticles was assessed using distinct techniques namely UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transmission infrared (FTIR) and High resolution- transform electron microscopy (HR-TEM). The antioxidant activity of aqueous extract and biosynthesized silver nanoparticles was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The different concentrations of sample ranging from (100-1000 µg/ml) was prepared and mixed with 3 ml of DPPH solution. The maximum antioxidant activity was confirmed at 1000 µg/ml of concentration that is 91 % and 88 %, respectively. The antimicrobial property of silver nanoparticles derived from S. mukorossi extract was evaluated against four strains viz., P. aeruginosa, E. coli, B. subtilis and S. aureus. The maximum antimicrobial property was seen against gram-negative bacterium P. aeruginosa, E. coli then gram-positive bacterium B. subtilis and S. aureus. These results suggest that silver nanoparticles made from S. mukorossi extract may beused as antimicrobial and antioxidant agents in several fields, including medicine, agriculture, and personal care products.