A PROTEOMIC APPROACH TO AUGMENT QUALITY OF CHICKEN MEAT THROUGH DIETARY NATURAL ANTIOXIDANTS
| dc.contributor.advisor | PARTHIBAN M | |
| dc.contributor.author | DIVYA MANJARI K | |
| dc.contributor.author | TANUVAS | |
| dc.contributor.other | KARUNAKARAN R | |
| dc.contributor.other | APPA RAO V | |
| dc.contributor.other | SENTHILKUMAR TMA | |
| dc.date.accessioned | 2024-01-02T15:24:39Z | |
| dc.date.available | 2024-01-02T15:24:39Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Probiotics were reported to improve body weight gain and feed efficiency and reduced mortality in broiler chicken. In continuation of using probiotics as performance enhancers in broilers prebiotics are also supplemented to enhance the activity of probiotics and also aid in scavenging free radicals that are responsible for creating stress in the mitochondria. Here in this study chicory is a natural antioxidant that is used as prebiotic as feed supplement. In this study a total of 21 probiotic organisms belonging to seven different species were isolated from chicken gut. These twenty one probiotic organisms were characterized by Gram staining, Biochemical methods using 21 sugars, pH tolerance and bile salt tolerance test, enzyme analysis using 19 different enzymes. These probiotic organisms were identified using molecular studies using PCR amplification of 16S -23S spacer region and sequencing analysis. Based on the sequencing data, seven different species were found to exist namely Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans, Lactobacillus salivarius. Bacillus subtillis and Pediococcus acidilactici. Based on extracellular and in vitro assays a scoring system was evolved to evaluate the potential probiotic bacteria with antioxidant property. These probiotic bacteria along with prebiotic chicory was supplemented in broilers and the performance was studied and observed that the live weight was increased by 200 grams in the synbiotic treated groups. The pH, meat tenderness and total protein were also improved in synbiotic treated groups. Also the proteome of the breast muscle was studied for differentially expressed proteins which relate to the enhancement of broiler performance in the experimental trial. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810205310 | |
| dc.language.iso | English | |
| dc.pages | 1-189 | |
| dc.sub | Animal Biotechnology | |
| dc.theme | Veterinary Science | |
| dc.these.type | Ph.D | |
| dc.title | A PROTEOMIC APPROACH TO AUGMENT QUALITY OF CHICKEN MEAT THROUGH DIETARY NATURAL ANTIOXIDANTS | |
| dc.type | Thesis |