Genome Wide Identification of "R" Genes and Exploition of Candidate RGA Markers in Mungbean (Vigna radiata) and Urdbean (Vigna mungo)
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Date
2011
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Publisher
UAS, Dharwad
Abstract
The present investigation was carried out with an objective to characterize the
NBS-LRR degenerate markers in urdbean and mungbean and thus to study the
polymorphism at RGA loci. Thirteen and twelve genotypes of mungbean and urdbean
were screened for MYMV, powdery mildew and CLS diseases. The genetic
variability was also studied. Similarly, genetic variability and molecular diversity was
studied in the germplasm lines of mungbean and urdbean in three different
experiments. None of the mungbean genotypes were found resistant to all the three
diseases while Uttara was found resistant to all the three diseases among the urdbean
genotypes. RGA1-TG and MtB99 were specific to MYMV. Selection based on
number of pods per plant, number of branches and bunches and total seed yield with
high GCV and PCV is effective. Very high heritability and genetic advance estimates
were recorded for all the characters studied among mungbean, unlike in urdbean,
where high heritability with high genetic advance estimates were recorded for plant
height, number of branches, number of bunches, number of pods per plant and total
seed yield indicating the role of additive gene action. Seed yield was found to have
non significant association with all the characters studied among the urdbean
genotypes. The genotype TAU7 formed a separate cluster from the rest of the thirty
nine genotypes making it an obvious choice in breeding program. Among the seventy
nine germplasm lines of mungbean seed yield per plant was significantly and
positively associated with number of pods per plant, number of seeds per pod and test
weight. The genotype Uttara can be used as one of the parents in resistance breeding
progamme. The two putative markers identified can be used to differentiate the
resistant and susceptible genotypes among mungbean genotypes and they could also
be further validated using mapping populations.
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Genetics and Plant Breeding