Optimization of extraction conditions for anthocyanin from Hibiscus rosasinensis and its characterisation by Chromatography techniques
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Date
2022
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Abstract
The present study was conducted to identify the better extraction conditions for anthocyanin from fresh and dry petals of hibiscus with potent antioxidant activity and polyphenol content. The variables used in this study are solvents (aqueous, ethanol, methanol and each added with acidified agents (1% citric acid, 0.1% HCl and 1% acetic acid), temperatures (40 ºC, 50 ºC, 60 ºC, 70 ºC and 80 ºC), steeping time (interval viz., 60min, 90min, 120 min, 150min, 180min and 210min and pH (1-9). The study revealed that the anthocyanin content was higher for fresh petals of hibiscus extracted with test solvent methanol acidified with 0.1% HCl with the mean and standard error values of 167.69±0.41 mg CAG/100g. From the results, the optimized condition for anthocyanin extraction chosen was at the temperature of 60ºC for 120min at pH of 3-3.5. However, for the food uses, fresh petals extracted with ethanol (acidified with 1% citric acid) was recommended. Their yield at optimized extraction condition was found to be 156.25 ± 0.13 mg CAG/100g with the total phenolic content of 4965.83±1.19 (mg./100g) and 75.33±0.33% antioxidant activity. The F-values for each response implied high significance of the fittest models. Thin Layer Chromatography (TLC) and Paper Chromatography (PC) chromatograms predicted that the major types of anthocyanin present in hibiscus petals were cyanidin and delphinidin.
Description
TNV_JPP_2022_11(2)128-132
Keywords
Veterinary Science