IDENTIFICATION OF QTLS ASSOCIATED WITH OIL CONTENT RELATED TRAITS IN SAFFLOWER (Carthamus tinctorius L.)

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dc.contributor.advisorROJA, V.
dc.contributor.authorNAVYA, RAVURI
dc.date.accessioned2022-08-17T08:50:35Z
dc.date.available2022-08-17T08:50:35Z
dc.date.issued2022-08-17
dc.descriptionIDENTIFICATION OF QTLS ASSOCIATED WITH OIL CONTENT RELATED TRAITS IN SAFFLOWER (Carthamus tinctorius L.)en_US
dc.description.abstractThe present study was conducted at Indian Institute of Oilseeds Research (IIOR), Rajendranagar, Hyderabad, Telangana, to identify the Quantitative Trait Loci (QTLs) associated with oil content related traits in safflower. Safflower is a valuable oilseed crop well-known for its high-quality edible oil. Though it contributes to meet the demands of edible oil in the country, the cultivation of safflower is rapidly decreasing due to its low productivity. Serious efforts are required to improve the oil yield potential in safflower to make it attractive to the farmers. Genetic enhancement of a quantitatively inherited traits like oil content requires simultaneous improvement of seed yield and oil content. Molecular mapping of genes or genomic regions associated with oil content or its related traits would help in enhancement of oil yield potential. In this context, the present study was conducted with to identify the quantitative trait loci (QTL) associated with oil content related traits using SSR markers in safflower. A set of 176 F2:3 progenies developed from a cross between A-1(low oil content, 26%) x EC-755673-1(high oil content, 37%) was used as mapping population. Polymorphism between the parents was analysed using 250 SSR loci which resulted in identification of a set of 30 polymorphic loci (12%) between the parents. The mapping population was genotyped using a set of 85 polymorphic markers including 60 polymorphic markers from the previous studies. The Chi-square (χ2) test revealed that, out of 85 polymorphic markers genotyped, 67 markers followed the Mendelian ratio of 1:2:1, whereas the remaining 18 markers deviated from the expected ratio. The genotypic data of 85 markers subjected to linkage analysis in JoinMap® version 3.0, which resulted in 17 linkage groups covering 83 markers and two ungrouped markers. The linkage map developed in this study provided the linkage information of a new set of 25 previously unmapped SSRs. However, the linkage map developed in this study is highly fragmented, mainly due to less number of markers screened. xiv Single marker analysis based on simple regression showed, 20 out of 85 marker loci were significantly associated with various seed traits located on different linkage groups identified in this study. The markers that significantly linked to different seed traits identified in the present study were CT12, CT40, CTDES265 (LG6) highly associated with seed length. The marker SAFM336 (UL1) was highly associated with seed breadth. The markers MCTIIOR89 (LG5a), SAFM10 (NA5), SAFM336 (UL1) and stp (UL2) were highly associated with seed thickness. The marker stp (UL2) was highly associated with L/B ratio. The markers MCTIIOR89 (LG5a) and stp (UL2) were highly associated with LBTP. The marker CT32 (LG5b) was highly associated with test weight. The markers CTDES91, CTDES72, CTDES81 (LG3a), CT40 (LG6), SAFM336 (UL1) and stp (UL2) were highly associated with oil content. Entire LG 6 associated with the seed length in the present study. Composite interval mapping (CIM) detected the putative genomic regions associated with these seed traits. The QTLs on LG5 (CAT85- MCTIIOR89, MCTIIOR89 - CT137) were common for seed thickness and LBTP. The QTL on NA5 (CAT18 - SAFM10) was associated with seed breadth, seed thickness and LBTP. SAFM336 was associated with seed breadth, seed thickness, L/B ratio and oil content. The putative QTLs for test weight (CTDES237 - CAT85, CT32 - CTDES92) and oil content (CTDES72 - CTDES81, SAFM336) were not common. Overall, the phenotypic variance explained (R2) by each QTL ranged from 4 to 24%. Most of the QTLs appeared to be minor while two QTLs, SAFM336 (seed thickness and L/B ratio) and CTDES72 - CTDES81 (oil content) showed larger R2 values (>20%) with LOD score 3.4 and 6.6, respectively. The results indicated the possibility of a potential major QTL for oil content on Linkage group-3a. The QTLs detected in this study can be considered as maiden QTLs for various seed traits in safflower. Considering the limitations encountered in the present study like population size, less marker coverage, lack of proper linkage map etc. the results need validation in a different mapping population.en_US
dc.identifier.otherD6260
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810186396
dc.keywordsIDENTIFICATION, QTLS , OIL CONTENT,TRAITS, SAFFLOWER (Carthamus tinctorius L.)en_US
dc.language.isoEnglishen_US
dc.pages126en_US
dc.publishergunturen_US
dc.subGenetics and Plant Breedingen_US
dc.themeIDENTIFICATION OF QTLS ASSOCIATED WITH OIL CONTENT RELATED TRAITS IN SAFFLOWER (Carthamus tinctorius L.)en_US
dc.these.typeM.Scen_US
dc.titleIDENTIFICATION OF QTLS ASSOCIATED WITH OIL CONTENT RELATED TRAITS IN SAFFLOWER (Carthamus tinctorius L.)en_US
dc.typeThesisen_US
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