SERODIAGNOSTIC STUDIES ON VIRUSES INFECTING APPLE
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Date
2010
Authors
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Journal ISSN
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Publisher
UHF,NAUNI,SOLAN
Abstract
ABSTRACT
Investigations were conducted to record the incidence of viruses in apple
orchards of the state along with serological detection by employing ELISA, besides
indexing of some trees of commercial cultivars of apple. Seventeen apple orchards
surveyed in Shimla, Kinnaur and Kullu district of HP revealed the presence of viral
infection with an incidence of 5-99 per cent. Both indirect DAC ELISA and direct
DAS ELISA assays resulted in detection of ACLSV, ApMV, ASGV and ASPV in
apple trees of different commercial cultivars being grown in different orchards.
ACLSV, ApMV, ASGV and ASPV were found to be present in serologically
detectable limit in leaves, bark and petals parts of an infected plant. However leaf
sample drawn during June, bark taken during August and petals taken during April
month found to contain highest relative concentration of these viruses based on the
OD values obtained in serological reactions. About 50 per cent of the diseased plants
revealed the presence of two or more than two viruses, thus indicating prevalence of
mixed infection. Out of 115 asymptomatic apple trees of different commercial
cultivars selected through viral indexing, 4 trees were found to carry latent infection
of ACLSV. Virus tested trees were marked to serve as mother trees for providing
budwood use in propagations.
Description
Keywords
economics, biological phenomena, resins, productivity, technological changes, exhibitions, developmental stages, marketing, biological development, selection