MOLECULAR CHARACTERIZATION OF VIRUS ASSOCIATED WITH YELLOW MOSAIC DISEASE IN HORSE GRAM
| dc.contributor.advisor | Dr. PRADEEP MANYAM | |
| dc.contributor.author | BODELA SUSHMA | |
| dc.date.accessioned | 2024-05-06T15:44:41Z | |
| dc.date.available | 2024-05-06T15:44:41Z | |
| dc.date.issued | 2024-05-06 | |
| dc.description.abstract | In this study, begomovirus associated with YMD of horsegram in Andhra Pradesh was studied based on diseased samples collected from Anantapuramu, Chittoor and YSR Kadapa districts. Based on PCR amplification and sequencing analysis, it was confirmed that all five samples infected with HgYMV indicating its predominance in horse gram growing areas of AP. Whole genome was determined for all the five isolates using overlapping primers for amplification of DNA-A and DNA-B components of HgYMV. The size of DNA-A in five isolates ranged from 2733-2736 nucleotides whereas for DNA-B, it was found to be 2670-2726 nucleotides in length. Complete genome analysis and sequence identity of HgYMV DNA-A and DNA-B of five isolates shared maximum nucleotide identity ranged from 95.5-98.7% for DNA-A and 94-98.2% for DNA-B with other HgYMV infecting pulses from different places. Among DNA-A and DNA-B components shared more than 94% identity among themselves, thus provining five isolates of HgYMV belong to same variant of the HgYMV. The recombination analysis revealed that DNA-A and DNA-B of HgYMV under present study is a recombinant, which is a derivative of known virus. The sequence analysis of IR region of HgYMV DNA-A and HgYMV DNA-B yielded broad range of identity (50.8-98.8% and 27.4-98.7% respectively) with HgYMV infecting other legumes. The IR region of all five isolates contains conserved TATA box, Nanomer and Ori sites. Nucleotide analysis of six ORFs of HgYMV DNA-A component with other begomoviruses was attempted. ORFAV1 and ORFAV2 shared 95.3 to 100% and 81.8 to 100% identity respectively with compared HgYMVs different isolates of HgYMV. A high similarity was also observed for other ORFs AC1 (95.0-100%), AC2 (80.7-100%), AC3 (83.5-99.2%) and AC4 xviii (92.8-100%). When compared with other begomovirus the closest association was found for MYMIV (80.6-86.6%), MYMV (82.2-84.5%) for DNA-A and MYMIV (60.3-68.4%), MYMV (60.5-68.3%) for DNA-B and less similarity was observed for DoYMV, VbGMV and FbLCV. The nucleotide analysis of ORFs of DNA-B component showed that high similarity (94.1-100%) was observed for BV1than BC1 (77.8-100%) which indicates BC1 is a divergent (KDP and TPT isolates). Bemisia tabaci populations were collected from four districts of Andhra Pradesh (YSR, Anantapuramu, Chittoor, Nellore) for identification biotypes and genetic groups. The existence of two genetic groups was revealed based on partial mtCOI gene sequence analysis. KDP and NLR belong to Asia-II-1 genetic group. Asia-II-8 genetic group was 1st time reported in AP identified in ATP and TPT B. tabaci population. The association of secondary bacterial endosymbionts with Bemesia tabaci populations was studied using16S rRNA/23S rRNA gene sequences. Wolbachia, a s-endosymbiont was identified in all four isolates which belongs to super group B. Whereas the association of Cardinium and Arsenophonus was observed from Kadapa B. tabaci population. The phylogenetic analysis identified Cardinium connected to C2 subgroup and Arsenophonus to A2 subgroup. Rickettesia was absent in all B. tabaci populations. Thus, Asia -II-1 genetic group from KDP was found in association with Wolbachia, Cardinium and Arsenophonus, while Asia-II-8 genetic group associated with only Wolbachia. A total of 37 Horse gram genotypes were screened for identifying resistance against Horse gram yellow mosaic virus. The PDI values of 37 genotypes ranged from 0-97.2%. Eighteen genotypes were found resistant under natural disease screening methods. Eight genotypes exhibited higher degrees of susceptibility (30.85-97.2%). The apparent rate of infection which depicts disease progression indicated that slow progress in resistant type genotypes (BSP21-3, r:0.20), whereas faster rate of spread disease in susceptible genotypes Indira Kulthi (r:0.45) and BSP21-5 (r:0.454). | |
| dc.identifier.other | D6564 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810208721 | |
| dc.keywords | MOLECULAR CHARACTERIZATION | |
| dc.keywords | YELLOW MOSAIC DISEASE | |
| dc.keywords | HORSE GRAM | |
| dc.language.iso | English | |
| dc.pages | 208 | |
| dc.publisher | Acharya N G Ranga Agricultural University | |
| dc.relation.ispartofseries | D6564; D6564 | |
| dc.sub | Plant Pathology | |
| dc.theme | MOLECULAR CHARACTERIZATION OF VIRUS ASSOCIATED WITH YELLOW MOSAIC DISEASE IN HORSE GRAM | |
| dc.these.type | M.Sc | |
| dc.title | MOLECULAR CHARACTERIZATION OF VIRUS ASSOCIATED WITH YELLOW MOSAIC DISEASE IN HORSE GRAM | |
| dc.type | Thesis |