Isolation and characterization of lipase producing bacteria
Loading...
Date
2006
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
Lipases are enzymes that are widely distributed among
microorganisms, animals and plants. However, microbial lipase are the
enzymes with diversified properties and substrate specification which make
them useful for industrial application. Lipase of pathogenic bacteria such as
Cornybacterium acnes, Mycoplasma, play a crucial role in the etiology of
diseases. Whereas some other bacterial lipases have been used in the
bioremediation of environmental problem, in domestic sewage etc. In addition
to this microbial lipases spoil the dairy and other foods containing fats, some
times even at refrigerated temperature. Lipase have role to play in detergent,
paper, cosmetic, and agrochemical industries. (Jagges et al., 1996). In view of
the importance and application of bacterial lipases the present study was
conducted.
Eight different samples from 4 different sources were collected
and 73 bacterial cultures that produced lipase were isolated on the basis of
formation of zone of clearance on solid medium. For further screening 29
isolates were selected and the enzyme activity was determined by titrimetric
method. The screening was also done by well method. Out of 29 isolates 5 i.e.
isolate No. 18, 21, 22, 47 and 48 showed better lipase activity than others.
Among the five isolates, No. 21 and 18 produced maximum lipase, 21.5
Units/ml and 18.3 Units/ml respectively and thus were selected for further
studies. Both the isolates produced maximum enzyme at 30ºC and pH 8.
Enzyme activity was maximum at 60ºC for isolate No. 21 and 50ºC for isolate
No. 18. The optimum pH for the activity of lipase from both isolates was
found to be 8.0. Under shake culture condition both the isolates produced
maximum amount of lipase than under stationary conditions. Glucose and
tryptone were the best sources of carbon and nitrogen respectively for
production of lipase in both the isolates. The cultural, morphological,
biochemical characteristics indicate that lipase activity is present in a variety
of bacteria.