Analysis of genetic diversity of Labeo rohita from selected zones of river Ganga and its comparison with cultured stock
Loading...
Date
2017-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was conducted to investigate the level of polymorphism and genetic diversity in natural population of Labeo rohita collected from selected zones of River Ganga (from Haridwar and Kanpur region) and also in the cultured population (from Instructional Fish Farm of College of Fisheries, Pantnagar) using microsatellite DNA markers. 5 primers out of 10 produced amplicons and were selected for primary screening and further genetic analysis of the population. On the basis of the values of Average Nei’s Genetic Diversity, observed and expected heterozygosity, fixation index, Shannon’s information index and cluster analysis obtained by microsatellite marker technique, the present study is the indicative of the fact that the stock of L. rohita of Haridwar region of River Ganga is more genetically diverse and genetically differentiated as compared to the stock of Kanpur region as these sites are distantly located and having negligible chance of exchange of gene pool with each other which is responsible for weak sub-structuring of stocks. Genetic variation observed between the two riverine sites may also be caused due to high level of anthropogenic activities in Kanpur region thereby causing blockage of fish movement as well destruction of breeding grounds thus hampering the proper mating within the population and also reducing effective population size. The present finding also indicates that L rohita samples collected from riverine system showed very high level of genetic diversity and negligible inbreeding in comparison to the population present in captivity. The lesser value of heterozygosity and increased inbreeding in population of L rohita from culture as compared to wild might be due increase in incidents of repeated breeding in successive generations owing to lack of regular germplasm exchange of appropriate genetic diversity. Genetic diversity analysis also revealed substantial changes in genetic variation and significant genetic distance between the wild and farm-produced populations of L. rohita.
Description
Keywords
null