ASSOCIATION OF SELECTIVE GENES FROM EARLY LACTATION STRESS SPECIFIC ADIPOSE TISSUE WITH POST-PARTUM ANESTRUS IN BUFFALOES
| dc.contributor.advisor | SUNEEL KUMAR ONTERU | |
| dc.contributor.author | SURYA KANT VERMA | |
| dc.date.accessioned | 2023-11-10T11:50:06Z | |
| dc.date.available | 2023-11-10T11:50:06Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Genetic markers associated with postpartum anestrus interval (PPAI) of buffaloes need an extensive exploration for implementing them in marker assisted selection programs. Therefore, the present study identified 29 SNPs in 11 candidate genes using pooled DNA of Murrah buffaloes with extreme positive (case) and negative (control) PPAI residuals resulted by adjusting the recorded PPAI with 38 nongenetic variables collected in the field conditions. Considering the conservative bioinformatics based functional criteria, only 7 SNPs located in the AKR1C3, PLG, MYF5, SEC14L2, TPM1, LAMA2 and TRHDE genes were selected for association analysis. During the discovery phase, the SNPs in LAMA2 (c.+549967C>A) and TRHDE (c.+445G>C) genes were significantly associated (P=0.02267 and P=0.0021, respectively) with PPAI in a population sample of 50 extreme cases and 50 controls. The significant association of the SNP in LAMA2 (c.+549967C>A) gene was further confirmed (P=0.0401) in a validation population of 415 buffaloes, in which the SNP explained 1.36% of genetic variance and the CC genotypes had 12.71 ± 3.21 PPAI days greater than that of AA and CA genotypes. Computational analysis showed the RNA transcribed from C allele of the LAMA2 (c.+549967C>A) gene might have higher stability than that of A allele. This prediction was confirmed by higher LAMA2 gene (P = 0.01798) expression (> 8 fold) and fat percentage (P < 0.05) in the adipose tissue of CC genotypes (48.78 ± 1.87%) than AA genotypes (33.59 ± 4.5%). Similarly, significantly (P<0.05) higher upregulation of the CEBPβ, PPARγ, FASN, CEBPα, PNPLA2 and the downregulation of BMP2 and PTCH1 genes in LAMA2 (c.+549967C>A) CC genotypes than AA genotypes, indicating the role of PPARγ mediated pathway for both the adipogenesis and lipolysis in CC genotypes. Overall, we found an association of the LAMA2 (c.+549967C>A) and TRHDE (c.+445G>C) SNPs with PPAI in Murrah buffaloes, which needs future validation in large populations. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810200916 | |
| dc.language.iso | English | |
| dc.pages | 135 p. | |
| dc.publisher | ICAR-NDRI, KARNAL | |
| dc.sub | Animal Biochemistry | |
| dc.theme | ANIMAL BIOCHEMISTRY | |
| dc.these.type | Ph.D | |
| dc.title | ASSOCIATION OF SELECTIVE GENES FROM EARLY LACTATION STRESS SPECIFIC ADIPOSE TISSUE WITH POST-PARTUM ANESTRUS IN BUFFALOES | |
| dc.type | Thesis |