Micropropagation Studies in Pomegranate (Punica granatum L.) CV. Kesar
| dc.contributor.advisor | Patil, S.S. | |
| dc.contributor.author | Malawadi, Vitthal S. | |
| dc.date.accessioned | 2021-10-03T08:49:47Z | |
| dc.date.available | 2021-10-03T08:49:47Z | |
| dc.date.issued | 2016-08 | |
| dc.description.abstract | An investigation carried out on micropropagation studies in pomegranate (Punica granatum L.) cv. Kesar during 2014-16 at the Tissue Culture Laboratory of Department of Horticulture, College of Agriculture, University of Agricultural Sciences, Dharwad. An efficient protocol developed for rapid shoot multiplication of pomegranate cv. Kesar using nodal segment derived from young and healthy plantlets. Shoot development was induced from nodal segments on Murashige and Skoog (MS) medium supplemented with BAP 0.6 and 1.0 mg l-1, NAA 0.2 and 0.4 mg l-1, kinetin 1.0 and 2.0 mg l-1 and BAP 0.6 mg l-1 + kinetin 1.0 mg l-1 and BAP 1.0 mg l-1 + kinetin 2.0 mg l-1 were cytokinin significantly induced shoot proliferation. The maximum number of shoots (5.63 shoots per explant) developed on a medium containing BAP 1.0 mg l-1 + kinetin 2.0 mg l-1. Shoot culture was established by repeatedly sub-culturing the original nodal segment on a fresh batch of the same medium after each harvest of the newly formed shoots. In vitro raised shoots were cultured on a fresh medium for further multiplication. Shoots formed in vitro were rooted on MS medium supplemented with NAA 0.5, 1.0 mg l-1, IBA 0.5, 1.0 mg l-1 and IBA 0.5 mg l-1 + NAA 0.5 mg l-1 and IBA 1.0 mg l-1 + NAA 1.0 mg l-1. However, a medium containing IBA 0.5 mg l-1 + NAA 0.5 mg l-1 resulted significantly in lesser number of days taken for rooting (21.50), more number of root per shoots (4.64) and highest root length (4.60 cm). In vitro rooted shootlets were successfully acclimated in coco-peat with a maximum survival (87.67 %) and finally established in soil: compost (1:1) medium. | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810176540 | |
| dc.keywords | Micropropagation Studies in Pomegranate (Punica granatum L.) CV. Kesar | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 52 | en_US |
| dc.publisher | University of Agricultural Science, Dharwad | en_US |
| dc.research.problem | Micropropagation Studies in Pomegranate (Punica granatum L.) CV. Kesar | en_US |
| dc.sub | Horticulture | en_US |
| dc.theme | Micropropagation Studies in Pomegranate (Punica granatum L.) CV. Kesar | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Micropropagation Studies in Pomegranate (Punica granatum L.) CV. Kesar | en_US |
| dc.type | Thesis | en_US |