MINERAL PHOSPHATE SOLUBILIZATION AND MOLECULAR DIVERSITY OF AZOSPIRILLUM
| dc.contributor.advisor | P.U KRISHNARAJ | |
| dc.contributor.author | VEENA, P. PATIL | |
| dc.date.accessioned | 2019-06-10T05:56:15Z | |
| dc.date.available | 2019-06-10T05:56:15Z | |
| dc.date.issued | 2007-01-02 | |
| dc.description.abstract | The aim of the present study was to isolate Azospirillum from different crops and assess their molecular diversity, screen these isolates for their activity and mobilize pqq containing construct into MPS negative isolates and study the expression in these native Azospirillum isolates. Thirty isolates were isolated from different crops. This work reports about the presence of Azospirillum even in medicinal plants endorhizosphere e.g., ashwagandha, stevia, and Vitex nigundo. The ability of the isolates to fix atmospheric nitrogen ranged from 10.12 to 19.22 mg N per g of malate. All the isolates produced lAA, expect five all produced GA. Among the 30 isolates screened, 12 were found to be MPS negative but complemented for MPS activity with PQQ supplementation, indicating that they lacked genes for PQQ synthesis. Among these A75 and A10 isolates showed MPS activity when PQQ was added into MSM and these were AmpR and Tef^. Hence, a construct pJSKlS containing Pqq synthase gene(s) with AmpR and Kan^ as.marker was mobilized into Azospirillum A75 and AlO. The resultant transconjugants showed slight solubilization on MSM medium than their respective wild types, indicating that the PQQ gene (s) in pMCG898 was not sufficient to complement MPS activity in Azospirillum isolates A75 and AlO. However, the transconjugants showed a significantly higher Pi release of 24 and 111 per cent Pi release respectively than their respective wild types in TCP broth. | en_US |
| dc.identifier.other | TH-8350 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810107897 | |
| dc.language.iso | en | en_US |
| dc.pages | 118 | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Banglore | en_US |
| dc.sub | Agricultural Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | MINERAL PHOSPHATE SOLUBILIZATION AND MOLECULAR DIVERSITY OF AZOSPIRILLUM | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MINERAL PHOSPHATE SOLUBILIZATION AND MOLECULAR DIVERSITY OF AZOSPIRILLUM | en_US |
| dc.type | Thesis | en_US |