Genetic characterization of root nodule isolates from clones of Dalbergia sissoo
| dc.contributor.advisor | Johri, B.N. | |
| dc.contributor.author | Samant, Mukesh | |
| dc.date.accessioned | 2019-06-20T09:19:27Z | |
| dc.date.available | 2019-06-20T09:19:27Z | |
| dc.date.issued | 2003-08 | |
| dc.description.abstract | Dalbergia sissoo (Shisham) is an important Leguminous nitrogen fixing tree of tropics. Legume-Rhizobium symbiosis is important for nitrogen fixation. Therefore, present investigation was undertaken to study the genetic diversity of rhizobial partner of Dalbergia sissoo based on biochemical and molecular tools e.g., 16 S ARDRA, IGS ARDRA, BOX PCR profiling and Nif H amplification. ARDRA of 16 S rDNA region of isolates from clones of Dalbergia sissoo (CPT 5 and CPT 6) resulted in placement of all six isolates in separate group from other authentic rhizobial and Shisham isolates. IGS ARDRA showed higher diversity within these isolates than 16 S ARDRA. UPGMA cluster analysis of combined IGS and 16 S ARDRA showed diversity within the isolates of CTP 5 (DS 5 1a and DS 5 1b) and CPT 6 (DS 6 1a, DS 6 1b, DS 6 1c and DS 6 1d) but they clustered in the same group. None of the isolate was identical to other authentic rhizobial strain and other Shisham isolates. BOX PCR profile showed some identical band pattern in all the isolates. All isolates were Nif H positive suggesting operation of nitrogenase. DNA sequencing of these isolates will permit taxonomic identification and further phylogenetic assignment. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810109344 | |
| dc.keywords | genetic analysis, characterization, roots, nodules, isolates, clones, Dalbergia sissoo | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 102 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Dalbergia sissoo | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | Characterization | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Genetic characterization of root nodule isolates from clones of Dalbergia sissoo | en_US |
| dc.type | Thesis | en_US |