RECOVERY OF CANINE OOCYTES BY SLICING METHOD
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Date
2013
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Abstract
Ovaries were collected from bitches undergoing routine ovario-hysterectomy and transported in physiological
saline at 35°C to 38°C within 2 to 3 h of collection. A total of 1616 oocytes were retrieved by slicing of 52 ovaries
with a recovery rate of 31.07 ± 3.47 oocytes per ovary. Among the collected oocytes 25.93, 43.07 and 31.00
percent of oocytes were graded as I, II and Ill respectively. The overall good quality oocytes (grade I and II COCs)
recovered from bitch ovaries by slicing method irrespective of reproductive cycle in the present study was 69.00
percent.