Molecular Detection of Field Isolates of Infectious Bursal Disease Virus Adapted to African Green Monkey Kidney Cells
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Date
2017-11
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Abstract
Infectious Bursal Disease (IBD) is an acute, highly infectious viral disease of young chicken. In the present study molecular detection of Infectious bursal disease virus was carried out from field samples (Bursa). A total 65 field samples were collected from different districts of Tamil Nadu and processed for detection by Reverse Transcriptase polymerase chain reaction (RT-PCR). The hypervariable region of VP2 gene encoding the neutralizing epitopes (633bp) was amplified. The very virulent samples were characterized by infecting in African green monkey kidney cells (Vero) upto 5 passages. The adaptation studies are carried out up to 120 hours to monitor the cytopathogenic effects (CPE) RT-PCR was carried out on cell culture adapted IBDV of every passage. The TCID50 per ml of the adapted virus at 5th passage was 1.58 x107.These findings concluded that the RT-PCR amplification of VP2 gene on bursal samples and characterization studies in African green monkey kidney cells would serve as a valuable tool for isolation and identification of IBDV for development of novel vaccines in future.
Description
TNV_IJTRD_2017_4(6)133-135
Keywords
Veterinary Science