PROTECTIVE EFFECT OF VITAMIN E IN DOXORUBICIN-INDUCED TOXICITY IN RATS
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Date
2010-08
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The present study was aimed to evaluate the protective effect of vitamin E in doxorubicin-induced toxicity in rats, which were divided into 4
groups and treated as follows: Group 1: sham control, 2: doxorubicin control, 3: doxorubicin + vit-E @150 mg/kg b. wt and 4: doxorubicin + vit-E @ 500 mg/kg b. wt Average body weights were recorded at weekly intervals and organ weights were recorded at the time of sacrifice. On 28th day, organs were collected for estimation of TBARS, protein carbonyls and GSH in tissue homogenates. Activity of Na+-K+ ATPase , Mg2+ATPase and CYP450 of liver, intra-testicular LDH, serum troponins, creatinine, LDH and AST were also estimated, besides haematology at the end of experiment on 28th day. Histopathology of heart, liver, kidney and testes was also studied at the end. Body weight gain, relative organ weight, RBC, WBC, Hb, PCV, GSH, CYP450, Na+/K+ ATPase and Mg2+ATPase were significantly (P < 0.05) decreased in doxorubicin group, while TBARS, protein carbonyls, serum LDH, intra-testicular LDH, serum AST, creatinine and serum troponins were significantly (P < 0.05) increased in group 2. Group 1 did not reveal any abnormalities on histopathology. Group 2 (doxorubicin) showed marked congestion and degenerative changes in heart, kidney, liver and testis. Vitamin E-treated groups (3 and 4) showed improvement in all the parameters studied, though it was marked with vitamin E @ 500 mg/Kg. From this study, it is concluded that doxorubicin induces toxicity to heart, kidney, liver and testes, and these effects can be reverted by vitamin –E administration in a dose-dependent manner.
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