IMPACT ASSESSMENT OF BACTERIAL BIOFORMULATION ON Trillium govanianum (Wall. ex Royle) UNDER INTEGRATED APPLE FARMING SYSTEM
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Date
2022-12
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UHF,NAUNI
Abstract
ABSTRACT
Among the list of critically endangered medicinal plants of Himalayan region, Trillium govaniaunm commonly
known as ‘Nag Chhatri’ is one of the most valuable among them. Due to its notable health benefits and rising demand, it is
now being traded illegally and causing overexploitation of this plant from natural habitats causing its inclusion in the list of
critically endangered medicinal plants. Malus×domestica Borkh, (apple), is one of the most widely cultivated and top most
cash crop og Himachal Pradesh. The present study is aimed to isolate and identify the potential growth promoting bacterial
endophytes from rhizome of T. govaniaum for cultivation and its establishment with apple under integrated farming system.
The collection of rhizome samples has been done from four different locations (Yulla, Meeru, Bari and Niachr) of Kinnaur
district of Himachal Pradesh. In total 107 bacterial endophytes were isolated from rhizome and roots of T. govanianum. All
isolates were screened for the PGP traits, P-solubilisation, siderophore, ammonia, HCN production and lytic enzyme
activity out of which 22 had been selected for secondary screening for drought tolerance. On the basis of secondary
screening three potential bacterial endophytes Ar 13, Ar17 and Ms5 were finally selected. The three in-house rhizobacterial
isolates i.e. YL3,YL11, YL12 and three selected endophytes i.e. Ar13, Ar17 Ms5 have been further selected for molecular
characterisation by 16S rRNA sequencing and were identified as Pantoea vagans Ar13, Microbacterium phyllosphaerae
Ar17, Pseudomonas mandeli Ms5, Alcaligenes faecalis subsp. Phenolicus YL3, Pantoea vagans YL11, and
Brevundimonas naejangsanensis YL12. Furthermore, the charcoal bioformulation for these particular endophyte bacterial
strains was prepared. The field experiment was conducted in farmers apple orchard for consecutive two years in 8-10 year
(Malus×domestica cv. Royal delicious) at three distinct agroclimatic locations i.e. Yulla Bari and Nichar of Kinnaur district
HP. The selected bacterial isolates were utilized individually, in consortia with reduced dose of fertilizer to examine the
favorable impact of bioformulation on Apple and T. govanianum growth characteristics. Comparing the inoculation with
bioformulations to the uninoculated control, present findings demonstrated a beneficial effect. In comparison to other
treatments on the various plant characteristics, the consortia of P. vagans+ M. phyllosphaerae +P. mandeli was ascertained
as best for successful cultivation, establishment and growth promotion of T. govanianum in all three sites and in case of
apple consortia of endophytes/consortia or rhizobacteria+80% NPK exerted the statistically significant effect for the growth,
yield and quality parameters of apple. The findings of this study are highly significant for developing bioformulations in
intercropping system