IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH LYCOPENE CONTENT AND OTHER CAROTENOIDS IN TOMATO
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Date
2007-08-13
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UNIVERSITY OF AGRICULTURAL SCIENCE, GKVK , BANGALORE
Abstract
Tomato fruit is an important source of micronutrients. Lycopene is a very efficient
antioxidant, neutralizes - the reactive oxygen species derived from free radicals.
Twenty eight varieties of tomato plants were grown following the standard cultivation
package for tomato used. In the present study, highest Iycopene content was present
in variety Ruchi (105.41lJg/g) and minimum concentration of Iycopene was found in
variety Tomato Stone (10.53lJg/g). In case of ~-carotene, ~-cryptoxanthin, Zeaxanthin
and a-carotene, the highest value was recorded in variety Ruchi (103.78, 109.35,
108.47 and 98.40 lJg/g). Single Marker Analysis revealed that OPC4950and OPC4300
were putatively associated with lycopene with positive Parameter Estimate. For the 0carotene,
OPC 4950 and OPC 4300,contributed more than 47% with positive Parameter
Estimate. For 13- Carotene, OPC 4950and OPC 4280 also found contributing more than
47% . For the J3-cryptoxanthin, OPC 4950 and OPC 4280, contributed at 47.21% with
positive Parameter Estimate . Also markers OPC 4950and OPC 4300contributed more
than 470/0 with positive Parameter Estimate in Zeaxanthin. In Stepwise Multiple
Regression Analysis three markers accounted for increased lycopene up to 45.96%,
OPC4950 showed maximum putative association with Iycopene. Two RAPD markers
OPA 8270 and OPS 4320 were found contributing about 30% for the increase in the 0carotene.
There were no markers which positively contributed to ~-carotene, ~cryptoxanthin
and zeaxanthin. Diversity among genotypes by Jaccard coefficient
ranged from 46% to 92% and differences among genotypes with correlation
coefficient ranged from 66% to 99.98% . These results showed that the association of
various markers with different traits using single marker analysis and stepwise
multiple regression analysis is a putative association and these markers are needed
to be validated using various mapping populations, and can be used as potential
markers in marker assisted selection for the improvement of tomato with respect to
Iycopene and other carotenoids.
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