VALIDATION OF IDENTIFIED QTLs IN RECOMBINANT INBRED LINES FOR STAGNANT FLOODING TOLERANCE IN RICE.

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dc.contributor.advisorGIRIJA RANI, M
dc.contributor.authorSRIKANTH KUMAR, G
dc.date.accessioned2020-03-04T08:22:59Z
dc.date.available2020-03-04T08:22:59Z
dc.date.issued2019
dc.descriptionD5960en_US
dc.description.abstractThe present study was conducted at Regional Agricultural Research Station (RARS), Maruteru, West Godavari district of Acharya N. G. Ranga Agricultural University (ANGRAU) during Kharif 2018-19 to validate identified QTLs in Recombinant Inbred Lines for stagnant flooding tolerance in rice, to study character association and also effect of one character on the other character for 19 characters. Phenotypic data were recorded for physiological traits (plant survival %, stem elongation at 30 DAT, stem elongation at 60 DAT, flag leaf length, flag leaf width, kneeing ability), lodging related traits (basal internode length, culm diameter, culm thickness, culm strength, per cent of lodging) and yield traits (plant height, days to 50 % flowering, ear bearing tillers plant-1, number of grains panicle-1, panicle length, spikelet fertility, 1000 grain weight and grain yield plant-1). The analysis of variance revealed the presence of significant differences among checks, checks vs genotypes for all 19 characters denoting variability among genotypes. The result implies that RILs exhibit superiority over the susceptible parent Indra for the characters studied. Screening of 167 RILs for stagnant flooding using augmented randomized block design revealed the survival of 146 RILs. Among them, two RILs (SF 7, SF 75) exhibited 100 % plant survival % and 93 RILs exhibited less than 50 % plant survival. These results indicated that plant survival % is essential to realize considerable yields under stagnant flooding. Character association studies revealed that stem elongation at 60 cm, flag leaf length, flag leaf width, basal internodal length, culm diameter, plant height, days to 50 % flowering, ear bearing tillers plant-1, number of grains panicle-1, panicle length, spikelet fertility, 1000 grain weight exhibited positive significant correlation with grain yield plant-1 denoting selection of these traits helps in realizing potential yields under stagnant flooding. Per cent of lodging and kneeing ability showed negative correlation with grain yield plant-1 suggesting that lower per cent of lodging, lower values of kneeing ability are essential to achieve higher yields under stagnant flooding. xii Path analysis revealed that all the yield parameters exhibited positive direct effect on grain yield, all the physiological traits except kneeing ability showed negative direct on grain yield and all the lodging related parameters except per cent of lodging expressed negative direct effect on grain yield. Selection of plants with higher plant survival %, moderate plant height and elongation, high tillering ability having wider and strong culms with longer panicles would be useful for varietal improvement under stagnant flooding. The QTL qSF10.1 affecting plant survival % under stagnant flooding was validated between flanking markers RM 304 and RM 6737 on chromosome 10 with a LOD score of 1.60 explained by phenotypic variance of 7.56 % and additive effect of 7.71 during kharif 2018-19. Fine mapping of previously identified QTL region (RM 304 – RM 6100) using more SSR markers resulted in reduction of QTL region from 166 to 62 kbp. These results implies that QTL region (RM 304 – RM 6737) confers for plant survival %. In addition to validation of QTL qSF10.1, we have also identified 17 QTLs for physiological, lodging related and yield traits with in QTL region. These results were supported by correlation of plant survival % with the physiological, lodging related and yield traits. Out of 17 QTLs, qSE10.1 for stem elongation at 30 DAT, qLOD10.1 for per cent of lodging, qNG10.1 for number of grains panicle-1 and qGY10.1 for grain yield plant-1 with higher values of phenotypic variation can be exploited as major QTLs for yield improvement apart from validated QTL qSF10.1 for plant survival under stagnant flooding.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810144191
dc.keywordsRECOMBINANT INBRED LINES, STAGNANT FLOODING TOLERANCE, RICE.en_US
dc.language.isoen_USen_US
dc.pages208en_US
dc.publisherAcharya N G Ranga Agricultural University, Gunturen_US
dc.research.problemVALIDATION OF IDENTIFIED QTLs IN RECOMBINANT INBRED LINES FOR STAGNANT FLOODING TOLERANCE IN RICE.en_US
dc.subGenetics and Plant Breedingen_US
dc.subjectnullen_US
dc.themeVALIDATION OF IDENTIFIED QTLs IN RECOMBINANT INBRED LINES FOR STAGNANT FLOODING TOLERANCE IN RICE.en_US
dc.these.typeM.Scen_US
dc.titleVALIDATION OF IDENTIFIED QTLs IN RECOMBINANT INBRED LINES FOR STAGNANT FLOODING TOLERANCE IN RICE.en_US
dc.typeThesisen_US
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