ISOLATION, IDENTIFICATION AND CULTURAL SENSITIVITY OF Escherichia coli FROM MILK AND MILK PRODUCTS.
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Date
2019-07-23
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CSKHPKV, Palampur
Abstract
In the present study, microbiological quality of milk and milk products was assessed by employing standard plate count and coliform count with special emphasis on Escherichia coli which is a food borne pathogen of public health importance. A total of 205 samples of milk and its products were screened for the presence of E. coli. Based on serotyping maximum prevalence was found in fermented milk products (22.86%), followed by cream based milk product (16.67%), paneer/ channa based product (13.64%), flavoured milk product (4.76%) and processed cheese products (2.70%). Among 17 isolates confirmed by serotyping, 15 isolates belonged to 9 different ‘O’ serogroups viz. O2, O7, O8, O26, O118, O121, O128, O145 and O157 while 2 were untypable strains. On the basis of PCR, out of 25 biochemically characterised isolates 23 were positive for E. coli. All the isolates were characterized in terms of antibiotic resistance/sensitivity. Antibiogram studies of the isolates revealed that 44% of the isolates were multidrug resistant with maximum isolates showing resistance to cefotaxime (88%), followed by cefuroxime (52%), amoxyclav and azithromycin (36%) while 96% sensitivity was observed against gentamycin. Due to ever increasing problem of antibiotic resistance, effect of both methanolic and aqueous extracts of pomegranate peels, rhododendron flowers and drumstick was evaluated against E. coli. The antimicrobial activity of aqueous and methanolic extracts of pomegranate peels and rhododendron flowers was found to be effective against E. coli at the concentration of 10 per cent and 15 per cent.
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