HEMATOPOIETIC PROGENITOR CELL CHARACTERIZATION IN CANINES
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Date
2018
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Abstract
Blood cells are responsible for constant maintenance and immune protection of every cell type of the body and this relentless and brutal work requires cells that have the greatest powers of self-renewal and are designated as Hematopoietic progenitor cells (HPCs). Peripheral blood stem cells in circulation have become the most common source of hematopoietic stem cells intended for transplantation after minimal manipulation. In vitro and in vivo animal studies have shown that the enriched CD34+marrow cells of different species can give rise to the multiple blood cell lineages and may provide long-term hematopoiesis, suggesting that CD34 could be regarded as a cell surface marker of primitive hematopoietic stem cells. This has made it possible to enrich HSCs from mice and human beings for molecular characterization and transplantation purposes. Homeobox (Hox), sonic hedgehog (SHH), and Wingless-type MMTV integration site family (Wnt) are known to modulate the self-renewal and expansion of hematopoietic progenitor/stem cells in humans and mice. Unlike cytokines, Hox, SHH, and Wnt are highly conserved among species from flies to humans but studies regarding the self-renewal and expansion of the HSC are extremely limited in dogs. Hence in the present research, a rapid CD34+population enrichment and immuno separation protocol has been standardized in canine blood. The expression of signaling molecules for self renewal and colony forming potential of the isolated CD34+ population of cells from Canine blood showed that they were cells with proliferation and self renewal potential.
Description
TNV_TH_MSC(M)16001_2018
Keywords
Veterinary Science