Phosphate solubilizing Trichoderma sp. in bamboo (Bambusa sp.) rhizosphere
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Date
2005-06
Authors
Pant, Aakanksha
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Trichoderma sp. is a well-known biological control agent but very little work has been done on the nutrient solubilization potential especially P-solubilization. Therefore, to describe Trichoderma haying dual potential i.e. antagonistic property against fungal phytopathogens as well as P-solubilization, the present investigation was carried out. A total of eight isolates of bamboo rhizosphere along with standard culture of Tharzianum were used in present study. All the isolates depicted a differential degree of in-vitro antagonistic potential against chick- pea wilt
pathogens viz., Sclerotium rolfsii and Rhizoctonia solani inhibiting mycelia] growth of the two pathogens in dual culture plates. The various isolates formed mycelium on PDA with differential intensities of sporulation. When tested for their in-vitro phosphate solubilization using TCP (5g/L) all the isolates were found to have immense potential but of varying degrees. All the isolates solubilized higher concentrations of TCP i.e., from 5 to 30 g/L. Extra-cellular acid-- and alkaline phosphatase enzyme activities were found to be higher than acid phospha lase.
Alkaline phosphatase enzyme activities were found to increase with increasing concentration of TCP (from 5 to 30 g/L). The isolate BFH-3 showed 7.06 and 11.2:5 U/ml enzyme activity (alkaline) at 5 and 30 g/L of TCP respectively. Isozyme analysis by activity staining depicted two isoforms of alkaline phosphatases produced by BRH•3 and BRP--1 in presence of 30 g/L TCP.
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