ANTAGONISTIC EFFECT OF BACTERIA ASSOCIATED WITH MARINE ASCIDIANS AGAINST HUMAN AND FISH PATHOGENIC BACTERIA

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dc.contributor.advisorDr. Sugumar, G
dc.contributor.authorRAJA PRIYANKA MARY, M
dc.contributor.otherDr. Velayutham, P
dc.contributor.otherDr. Sundaramoorthy, B
dc.date.accessioned2017-06-16T05:36:46Z
dc.date.available2017-06-16T05:36:46Z
dc.date.issued2011
dc.description.abstractThe antagonistic properties of marine ascidians and the bacteria associated with them were investigated. Solitary and colonial ascidians were collected from submerged structures along the coast of Thoothukudi and were characterized. Solitary ascidians were identified as Cnemidocarpa areolata, Herdmania momus, Microcosmus exasperatus, Phallusia nigra and Polycarpa sp. Colonial ascidians were Didemnum psammathodes, Diplosoma swamiensis, Eudistoma viride and Lissoclinum fragile. The bacteria associated with the surface and whole body homogenate of selected ascidians were studied quantitatively and qualitatively. Epibiotic bacterial load varied from 3.0 × 102 to 8.4 × 105cfu/sq.cm., while the bacterial load from whole body homogenate of ascidians ranged from 5.7 × 104 to 1.2 × 107 cfu/ g. A total of 590 bacterial isolates were characterized up to generic level. The generic composition was dominated by Bacillus and Arthrobacter. All the bacterial isolates were tested for antagonistic property against indicator bacteria and 71 isolates were recognized as antagonistic, however, only 9 of them could effectively inhibit test organisms that included Escherichia coli (MTCC 443), Salmonella Paratyphi (MTCC 735), Salmonella Typhi (Clinical isolate), Pseudomonas aeruginosa (MTCC 27853), Vibrio cholerae (Clinical isolate), Vibrio harveyi (Shrimp larval isolate) and Staphylococcus aureus (MTCC 96). The inhibitory activity of the crude extracts of extracellular products (ECP) of selected antagonistic bacteria was weak against test organisms, but the ethyl acetate crude extracts of both cells and cell free supernatants of antagonistic bacteria showed strong inhibitory activity. Extracts from some of the antagonistic bacteria (DS13, DP41 and P19) showed a high degree of inhibition with zone of inhibition of ≥30mm dia against most test organisms. Methanol crude extract of one of the ascidians, Eudistoma viride showed good inhibitory activity against test organisms. The inhibition was strong against Vibrio cholerae and V. harveyi with zone diameter of 26 and 28 mm respectively. The MIC and MCC levels against V. cholerae and V .harveyi were also very low suggesting a very strong antibacterial activity. The results have clearly demonstrated that some ascidians produce strong antibacterial compounds and bacteria from marine sources have good antagonistic activity. These bioactive compounds could be harnessed for newer drugs in therapeutical applications.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810020764
dc.language.isoenen_US
dc.pages95en_US
dc.publisherFisheries College and Research Institute, Thoothukudi, Tamil Nadu Fisheries Universityen_US
dc.subFish Processing Technologyen_US
dc.subjectnullen_US
dc.themeANTAGONISTIC EFFECT OF BACTERIA ASSOCIATED WITH MARINE ASCIDIANS AGAINST HUMAN AND FISH PATHOGENIC BACTERIAen_US
dc.these.typeM.Scen_US
dc.titleANTAGONISTIC EFFECT OF BACTERIA ASSOCIATED WITH MARINE ASCIDIANS AGAINST HUMAN AND FISH PATHOGENIC BACTERIAen_US
dc.typeThesisen_US
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