STUDIES ON BIOCHEMICAL COMPOSITION OF FOLLICULAR FLUID AND OOCYTE CHARACTERlSTlCS IN BUFFALOES
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Date
2007-02
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
Studies on biochemical composition of follicular fluid and oocyte
characteristics in buffaloes were undertaken at Department of Animal
Reproduction, Gynaetxllogy and Obstetrics. College of Veterinary Science, '
Rajendranagar, Hyderabad-30. A total of 890 ovaries were collected from
January to August 2006 and used for the present investigation.
The average measurements of length, width, height, weight and sue of
left ovary were 2.04f0.05, 1.27f0.04, 1.41f0.04 crn, 2.72f0.15 g and
2.25a.21 cc. respe&ively and corresponding values for the right ovary truere
2.38fi.07, 1.44a.07, 1.63M.08 ern, 3.74M.28 g and 3.3W.36 cc,
mpectively. The mean number of Mile follicles on the surface of left and right
ovary was 3.87f0.23 and 3.66~.28.*~mheea n length, diameter and weight
of corpus luteurn were l.lOM.OB, 0.86M.04 cm and 0.88iO.W g,
respectively. The biochemical indices in follicular fluid of various sized follicles were
estimated in the present study as follows. Total protein (10.61~0.46g per
cent), albumin (2.95M.17 g per cent), globulin (7.6QM.39 g per cent),
albumin: globulin ratio (0.51fl.06 g per cent), cholesterol (48.36e.05 mg per
cent) and alkaline phosphatase (13.05M.59 KAU).
The mineral concentration in follicular fluid of different sized follicles
was as follows. Calcium (5.79iO.33 mgtdl), phosphorous (14.26M.27 mgldl),
sodium (82.25k1.85 ppm), copper (0.57i0.05 ppm), zinc (6.1 1f0.41 ppm) and
iron (1.35iO.05 ppm). The total number of oocyte yield in aspiration, dissection and slicing
techniques were 3.20M. 16, 3.10s. 10 and 5.70iO. 13, respectively. The total
number of oocyte yield in follicular and luteal phases of estrous cycle was
5.31a.12 and 3.88iO.13, respectively. Among the total oocytes recovered 52.94, 25.88 and 21.18 per cent were good, fair and poor quality of oocytes ir! the present study. The mean
diameter of good, fair and poor quality of oocytes were 135.65k2.52,
11 1.732.84 and 94.55Q.22 p, respedively. It was conduded that the bbmeUh of ovary and corpus luteurn were variable during the stages of estrous cycle and the number of follicles present on the ovary was not significantly #ired among the phases of estrous cycle.
It was also concluded that the biochemical and mineral content in
follicular fluid of buffaloes was playing a major role as the size of the follicle
increases and the mean number of oocytes retrieved was higher in slicing
technique and form the ovaries under luteal phase of estrous cyde.
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