CHARACTERIZATION OF GROUNDNUT TRANSGENIC PLANTS CO-EXPRESSING THREE REGULATORY GENES TO IMPROVE DROUGHT RESISTANCE
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Date
2017-09-11
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Adaptation of groundnut crop under rain fed conditions can be achieved by
improving suitable plant traits such as efficient water mining and cellular level tolerance
(CLT) mechanisms. Pyramiding these drought adaptive traits by simultaneous expression
of genes regulating root growth and sustaining cell metabolism by maintaining protein
turnover has phenomenal relevance in improving stress. From this context, Groundnut
transgenic plants expressing Alfin1, a root growth-associated transcription factor gene,
heat-shock factor (PgHSF4) and PDH45 involved in protein turnover and protection have
been developed. Emphasis in the present study is to identify superior promising events
that possess higher root growth, tolerance and productivity. The transgenic plants showed
more root growth at different developmental stages under both stress and non-stress
conditions. Further, the transgenic plants exposed to diverse stresses like salinity, DTT
and moisture stress, showed significantly superior performance compared to wild type.
The productivity of transgenic lines was more compared to wild type under both stress
and non-stress conditions. Based on stress tolerance with improved root growth and
productivity eight promising lines were identified. Further, to decipher the molecular
mechanism associated with improved root growth and tolerance the transcriptome,
proteome and metabolome analysis was done in wild type and transgenic plant. From the
transcriptome data it is evident that many root related genes and several heat shock genes
were up regulated in transgenics under stress condition. In proteomics data, several root
related and CLT associated proteins were up regulated. The metabolic profiling is
integrated with proteomic and transcriptome data which provided basic leads in
identifying key pathways contributing for improved adaptation, tolerance and
productivity under stress condition. The data clearly demonstrates that over-expression of
two or three regulatory genes improve drought adaptation by improving water relation
and CLT mechanisms.
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