Assessment of genetic stability of in vitro regenerated plantlets of Chlysanthemum morifolium L.
| dc.contributor.advisor | Reena Kumari | |
| dc.contributor.author | Sharma, Neeraj | |
| dc.date.accessioned | 2024-04-25T10:48:51Z | |
| dc.date.available | 2024-04-25T10:48:51Z | |
| dc.date.issued | 2024-04-08 | |
| dc.description.abstract | The present investigation was carried out on "Assessment of genetic stability of in vitro regenerated plantlets of Chlysanthemum morifolium L". Nodes, leaves, shoot tips and ray florets from maintained mother plants of chrysanthemum were used as the source of explants. These explants were treated with 5percent bavistin for l min followed by 0.3 percent sodium hypochlorite for 60 sec proved to be best as it gave 87.0 percent survival rate of nodal explant. 0.2 percent mercuric chloride for 30 sec proved to be best as it gave 83.0 percent survival rate of leaf explant. 0.1 percent mercuric chloride for 30 sec proved to be best as it gave 87.0 percent survival rate of shoot tip explant and 0.1 percent mercuric chloride for 60 sec proved to be best as it gave 85.0 percent survival rate of ray floret explant. Surface sterilized explant was culture on MS medium supplemented with different growth regulator and maximum in vitro shoot regeneration 92.0 percent from nodal explant was observed on MS medium supplemented with 1 .0 mg/I BAP and 1.0 mg/I NAA. However, Maximum direct shoot regeneration 85.0 percent was observed using shoot tip explant on MS medium containing 2.0mg/I NAA + 0.5mg/1 TDZ. Further MS medium fortified with 0.5mg/I BAP + 2.0mg/l IAA proved to be the best and showed 86.0 percent indirect shoot regeneration in case of ray floret explant. Whereas, MS medium fortified with l .5mg/1 BAP + 0.3mg/l 2, 4-D was reported to show 98.0 percent indirect shoot regeneration frequency from leaf explant. In vitro raised rnicroshoots were multiplied further on MS medium containing l .Omg/1 NAA+1 .0mg/1 BAP. Microshoots cultured on half strength MS media supplemented with 0.5mg/1 IBA showed 90.0 percent rooting after 3-4 weeks. Well developed in vitro raised plantlets were hardened in potting mixture containing cocopeat: perlite in 1:2 ratio with 85.0 percent survival rate. The genetic fidelity of Chrysanthemum morifolium L. clones was assessed by using SCoT (Start codon targeted) marker. Among 33 SCoT primers, 21 primers showed good scorable bands. The average number of monomorpbic bands per primer was 3.33 and with overall 96.16 percent monomorphism . The similarity coefficient values was ranged from 0.96 to 1.00 for SCoT markers. The results revealed that SCoT primer showed overall good monomorpbism (96.19) and six in vitro raised plants out of nine were found to be 100 percent similar to mother plant showing true to type nature of in vitro regenerated Chrysanthemum Morifolium L. plants. Hence, this protocol could be used for the large-scale production of true to type planting material of Ch1ysanthemum morifolium L. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810208380 | |
| dc.keywords | Plantlets | |
| dc.keywords | Chrysanthemum | |
| dc.keywords | Start codon targeted (SCoT) | |
| dc.keywords | Molecular marker | |
| dc.keywords | Biotechnology | |
| dc.language.iso | English | |
| dc.pages | 72+Vi | |
| dc.publisher | College of Horticulture & Forestry Dr YSP UHF, Neri, Hamirpur(H.P.) | |
| dc.sub | Biotechnology | |
| dc.theme | Genetic stability of in vitro regenerated plantlets of Chrysanthemum | |
| dc.these.type | M.Sc | |
| dc.title | Assessment of genetic stability of in vitro regenerated plantlets of Chlysanthemum morifolium L. | |
| dc.type | Thesis |