QUANTIFICATION OF PHORBOL ESTERS IN JATROPHA SEEDS, OILCAKE, BIODIESEL AND METHODS TO AMELIORATE TOXICITY LEVELS
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Date
40583
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The seed samples were collected from germplasm collection present
in GKVK, Bengaluru. Seed yield of 20 accessions ranged from 51 to
457.5 g, oil content ranged from 21 to 40 per cent and phorbol esters
were found using HPLC which ranged from 0.02-3.97mg/g. Out of 20
accessions, 6 contained phorbol esters less than 0.1mg/g, which is
considered as non-toxic accessions. Both toxic and non-toxic accession
of Jatropha curcas were found in southern Karnataka. Different
treatments were given to detoxify the oil which contained the highest
amount of phorbol ester (3.08 mg/g) out of which methanol treated
sample gave the best result (1.44 mg/g). The untreated oilcake contained
highest amount of phorbol esters (3.07 mg/g). This was reduced by
different treatments and among them oilcake treated with hydrochloric
acid was found to be the best (0.09 mg/g) treatment along with sodium
hydroxide followed by 92 per cent methanol wash in reducing phorbol
ester levels. Biodiesel also contained highest amount of phorbol esters
which was higher than oil and oilcake (5.38 mg/g) which was reduced by
bleaching (2.39 mg/g). The chemical composition of the oilcake after
different treatments was analysed to know the nutritive value.
Detoxification of oilcake was done to see that it could be used as a
supplementary feed stock for animals and also as protein supplement in
human diet. Though there was success in detoxification of cake to a
great extent, further refinement is needed.