In vitro propagation, elicitation and phytochemical characterization of Rheum emodi Wall. ex Meisn. (Himalayan Rhubarb)
| dc.contributor.advisor | Chaturvedi, Preeti | |
| dc.contributor.author | Singh, Ruchi | |
| dc.date.accessioned | 2019-08-30T10:57:59Z | |
| dc.date.available | 2019-08-30T10:57:59Z | |
| dc.date.issued | 2019-06 | |
| dc.description.abstract | Rheum emodi Wall ex. Meisn (Family- Polygonaceae) is an important folk medicinal herb of Himalayan Region. Underground parts (roots and rhizome) of the plant possess anticancer, antifungal, antidiabetic, antiulcer, antioxidant, nephroprotective and hepatoprotective properties. Being highly medicinal, the species is constantly uprooted from the nature for trade. As a result, the availability of the species in its natural habitat is decreasing. Besides, propagation through seeds is also very poor. The present study was henceforth aimed to enhance its seed germination potential, to develop an efficient, rapid and reproducible protocol for in vitro establishment along with enhancing secondary metabolites production by using various elicitors. Among all in vitro treatments applied for increasing seed germination, chilling treatment with alternate (16/8hr white light:dark) light condition on filter paper at 20ºC was found most effective to improve seed germination. Maximum callus induction frequency was achieved on MS + Kn+ 2,4-D+ TDZ and MS + BAP + 2,4-D + NAA from mid-rib and leaf explants respectively. MS + BAP + NAA+ IAA showed highest frequency of shoot induction from callus in 19 days with 9.0 number of shoots per explant. Maximum direct shoot induction frequency from leaf explant was achieved on MS medium fortified with BAP + TDZ + IBA in 14 days. Microshoots inoculated on MS + IBA+ NAA + BAP showed maximum frequency of root induction with 11.0 ± 0.33 roots in 23 days. Biochemical analysis of in vivo and in vitro raised plants showed that DPPH radical scavenging activity, TAA, total phenolic content and total protein content was highest in in vivo rhizome while TFC was highest in in vivo shoots. In vitro callus showed highest SOD, CAT and POD activity. Further, GCMS analysis showed 31, 38, 27 and 47 compounds in methanol extracts of rhizome, fruits, leaf and callus respectively. Among all tested elicitors (JA, SA and CH), SA(100 μM) was found most effective for enhancement of both enzymatic and non-enzymatic activities in callus of R. emodi. An effort was also made for root culture of R. emodi on both solid and liquid media. Liquid culture was more effective for production of adventitious roots with 93.33 ± 1.67 % root induction frequency from nodal explant along with highest DPPH radical scavenging activity, TAA, TPC and TFC. HPLC-MS analysis of cultured roots also revealed the presence of important anthraquinones viz., emodin and chrysophanol. The present study recommends the in vitro grown shoots and liquid root culture of R. emodi as an alternative source of anthraquinones relieving the dependence on natural population. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810125147 | |
| dc.keywords | in vitro culture, vegetative propagation, elicitation, phytochemistry, characterization, Rheum emodi, Himalayas, Rhubarb | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 218 | en_US |
| dc.publisher | G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand) | en_US |
| dc.research.problem | Rheum emodi | en_US |
| dc.sub | Botany | en_US |
| dc.subject | null | en_US |
| dc.theme | Phytochemistry | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | In vitro propagation, elicitation and phytochemical characterization of Rheum emodi Wall. ex Meisn. (Himalayan Rhubarb) | en_US |
| dc.type | Thesis | en_US |