GENETIC IMPROVEMENT IN POMEGRANATE (Punica granatum) THROUGH INDUCED MUTAGENESIS
| dc.contributor.advisor | Rajesh Kumar Dogra | |
| dc.contributor.author | MUNMUN JOSHI | |
| dc.date.accessioned | 2024-07-27T08:46:00Z | |
| dc.date.available | 2024-07-27T08:46:00Z | |
| dc.date.issued | 2024-02-04 | |
| dc.description.abstract | The contemporary study entitled “Genetic improvement in pomegranate (Punica granatum) through induced mutagenesis” was carried out during 2021-2022 at Department of Fruit Science, Dr YS Parmar University of Horticulture & Forestry, Nauni, Solan. The three cultivars viz., Kandhari Kabuli, Bhagwa and Daru were treated with physical mutagen (gamma rays) at doses of 0, 6, 9, 12, 15, 18, 21, 24 kR with the objective of inducing variability. The observations were recorded at different intervals of time after sowing in the polyhouse and after transplanting in the field conditions (20, 30 and 40 days after sowing and 70 days after transplanting, subsequently). The germination percentage, survival percentage and LD50 doses were calculated for all the three cultivars. Mutant seedlings from different concentrations of gamma rays were evaluated for morphological, biochemical and molecular characteristics. The morphological changes were observed both under polyhouse and field conditions. The LD50 dose for cvs. Kandhari Kabuli, Bhagwa and Daru were 15.26 kR, 15.08 kR, and 13.74 kR, correspondingly. Morphological characterization was done on the basis of the UPOV descriptor for the traits; plant: growth habit, plant: intensity of grey colour of main branches, shoot length (cm), young shoot: predominant number of leaves per node, leaf blade: length (cm), leaf blade: width (cm), leaf blade: ratio length/width, Leaf blade: shape of apex excluding tip; leaf blade: intensity of green colour; petiole: length; petiole: anthocyanin colouration. The disease severity was also screened for bacterial blight under field conditions, where, cv. Daru recorded the minimum disease severity percentage (6.57%) corresponding to control, followed by cv. Bhagwa (9.03%) and cv. Kandhari Kabuli (22.97%) at 9 kR. The molecular characterization was employed using 26 SSR markers to check the variability within the mutagenized seedlings with the Polymorphic Information Content (PIC), the effective multiple ratio (EMR) and the marker index (MI) aligned from 0.08 to 0.58; 0.00 to 0.17 and 0.00 to 0.06, sequentially. From the present study gamma rays induced variability for morphological traits will serve as a potential breeding material for future use. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810212535 | |
| dc.keywords | GENETIC IMPROVEMENT IN POMEGRANATE (Punica granatum) THROUGH INDUCED MUTAGENESIS | |
| dc.language.iso | English | |
| dc.pages | 92+iv | |
| dc.publisher | UHF Nauni | |
| dc.sub | Fruit Science | |
| dc.theme | “Genetic improvement in pomegranate (Punica granatum) through induced mutagenesis” | |
| dc.these.type | Ph.D | |
| dc.title | GENETIC IMPROVEMENT IN POMEGRANATE (Punica granatum) THROUGH INDUCED MUTAGENESIS | |
| dc.type | Thesis |