GENETIC DIVERSITY OF WHITEFLY VECTOR AND TOMATO LEAF CURL VIRUS INFECTING TOMATO AND THEIR INTEGRATED MANAGEMENT
Loading...
Files
Date
2018-09-24
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Agricultural Sciences, GKVK Bengaluru
Abstract
Whitefly (Bemisia tabaci) population and Tomato leaf curl virus (ToLCV) isolates
collected from different locations of Karnataka including endemic region of Maharashtra
(Narayanagaon) and subjected for phylogenetic analysis. The partial genome sequencing
(~1200 bp) of ToLCV revealed the presence of six species ToLCV viz., Tomato leaf curl
Bangalore virus (ToLCBV), Tomato leaf curl Kerala virus, Tomato leaf curl Joydebpur
virus (ToLCJoV), Tomato leaf curl Karnataka virus, Tomato leaf curl New Delhi virus
(ToLCNDV) and Tomato leaf curl virus. The ToLCBV was more common (40.43 %) in
both the endemic areas, whereas ToLCVND found only in Narayanagaon region.
Phylogenetic analysis of mtCO1 gene (~866 bp) sequences of whitefly population revealed
the presence of five genetic subgroups viz., Asia I India (more common - 65.50 %),
MEAM1, Asia II 5 Bangladesh Joydebpur, Asia II 8 India and Asia II 5 India (less common
- 3.45 %). Highly invasive MEAM1 whitefly population only detected dominantly in
Narayanagaon region (84.19 %). In Kadur region, both ToLCJoV and its probable specific
vector (Asia II 5 Bangladesh Joydebpur) were detected. Six segregating population
(COHBTTF1/11-4, COHBTTF1/86-4, COHBTTF1/99-2, COHBTTF1/99-5,
COHBTTF1/132-1 and COHBTTF1/253-1) from cross DMT2 X COHBT24 consistently
recorded resistance reaction, whereas COHBTTF1/95-7 recorded slow progression of
disease both under glasshouse and field screening studies. Among the four modules
evaluated, the newly formulated ultra-integrated module (UIM) comprising use of virus
free seedlings, barrier crop (maize), inter-cropping with marigold-yellow flower, foliar
application of Streptocycline, Metarhizium anisopliae, silicon, IIHR Vegetable special,
crude neem oil, and use of both stationary and mobile yellow sticky traps found effective
in significantly reducing whitefly population, per cent incidence and per cent disease index
of disease, whereas found safe for natural enemy like general predator, Chrysoperla
zastrowi. The UIM module recorded highest gross and marketable fruit yield during both
summer 2017 and 2018.