GENETIC DIVERSITY OF Y CHROMOSOME IN PUNGANUR CATTLE
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Date
2021-06
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
Punganur is a miniature cattle originated from Chittoor district of Andhra
Pradesh. Studies so far aimed at characterization of the breed at phenotypic level
and molecular level using autosomal microsatellite markers and mitochondrial DNA.
In addition to autosomal microsatellite markers and mitochondrial DNA, the Ychromosomal
variation also plays a key role in understanding population admixture
to trace paternal lineage. Differentiation of paternal lineages via analysis of Ychromosomal
variation adds significantly to what can be inferred from mtDNA and
autosomal variation in cattle. So far, the Y-chromosomal markers of Punganur cattle is
not explored.
Hence, the present study is proposed with the following objectives.
Objectives of investigation:
1. To characterize Y- chromosome of Punganur cattle using Y chromosome
specific markers
2. To determine Y chromosome haplogroups in Punganur cattle
A total of 60 Punganur bulls covering different geographical regions in and
around Andhra Pradesh and Telangana were screened to identify four Y chromosome
specific SNPs (ZFY9:120>C/T; USP9Y: 76426>C/T, SRY: 1748>G/T and UTY19:423>G/T). To detect these SNPs, a PCR-Restriction Fragment Length Polymorphism
(RFLP) strategy was designed for screening three SNPs (USP9Y: 76426>C/T, SRY:
1748>G/T and UTY19: 423>G/T) and Allele specific PCR method was adopted for
screening SNPs in ZFY9 (120>C/T) in the present study. The resulting restriction
profiles and Allele specific genotype were combined to yield haplotype (TCTT) specific
for Punganur cattle representing Y3 haplogroup of cattle. These results indicated that all
the animals in the present study belong to Y3 haplogroup which underpin the purity of
paternal lineage of Punganur cattle. The Y chromosome specific haplotype combination
(TCTT) deciphered in our study can be exploited as a marker in Punganur breed
conservation and improvement studies for detecting the purity/indigenous origin of paternal lineages
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