DETECTION OF ANTIBIOTIC RESISTANCE, GENETIC DIVERSITY IN Klebsiella pneumoniae ISOLATED FROM FOODS OF ANIMAL ORIGIN AND ITS PUBLIC HEALTH SIGNIFICANCE

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dc.contributor.advisorSUBHASHINI .N (MAJOR)
dc.contributor.advisorSRINIVASA RAO .T
dc.contributor.advisorASWANI KUMAR .K
dc.contributor.authorVENKATALAXMI ADIMULAM
dc.date.accessioned2024-06-21T12:01:49Z
dc.date.available2024-06-21T12:01:49Z
dc.date.issued2023-03
dc.description.abstractThe present study was undertaken to characterize K. pneumoniae in foods of animal origin and human clinical samples based on cultural and molecular techniques. A total of 402 samples comprising different foods like milk, meat, egg and fish, human clinical samples such as blood, urine and nasal swabs, rectal/cloacal swabs of pigs and chickens were analyzed for the presence of K. pneumoniae. The overall occurrence of K. pneumoniae was found to be 6.47% (26/402) by cultural and molecular methods in samples obtained from different sources. Out of 26 K. pneumoniae isolates, highest occurrence was found in urine samples (7/26, 15.21%) followed by pork (6/44, 13.64%), eggs (2/15, 13.33%), milk (5/58, 8.62%), chicken (2/42, 4.76%) and blood (4/102, 3.92%) samples. Phenotypic virulence factors such as capsule, hypermucoviscosity and biofilm formation were detected in 26 (100%), 11(42.31%) and eight (30.77%) isolates, respectively whereas virulence genes such as uge, rmpA and Kfu were observed in eight (30.77%), four (15.38%) and three (11.54%) isolates, respectively. None of the isolates carried the aerobactin gene. Antibiogram profiling of K. pneumoniae isolates indicated the highest resistance to erythromycin, vancomycin and ampicillin in 100%, 100% and 92.3%, respectively followed by cefpodoxime (80.77%), amoxicillin-clavulanic acid (76.92%), cefixime (57.69%), ceftriaxone (34.61%). All the isolates were sensitive to imipenem followed by piperacillin-tazobactam (84.61%). Intermediate resistance was observed to colistin (69.23%). All K. pneumoniae isolates were found to be MDR and MAR index value ranged between 0.18 to 0.65. ESBL production was confirmed in 14 K. pneumoniae isolates by both phenotypic and molecular methods and blaOXA, was the predominant gene which was found in eight among 14 isolates, blaCTX-M1 and blaCTX-M9 were found in four and two isolates, respectively and the β-lactamase gene blaAmpC was detected only in four isolates. A greater degree of heterogeneity was observed among 14 ESBL-positive K. pneumoniae isolated from different sources as revealed by presence of 14 genotypes each by ERIC and REP-PCR analysis. All the 14 different K. pneumoniae subtypes were differentiated by ERIC-PCR and REP-PCR. Genotyping of ESBL-producing K. pneumoniae by ERIC-PCR and REP-PCR was found to be highly significant since the discriminatory power >0.9 is considered highly significant (one for both ERIC-PCR and REP-PCR). Cluster analysis also revealed a great degree of heterogeneity and also the homogeneity among different isolates recovered from different sources thereby indicating that there is a chance of cross-contamination between various foods of animal origin.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810210726
dc.language.isoEnglish
dc.pages187
dc.publisherSRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
dc.subVeterinary Public Health
dc.themeDETECTION OF ANTIBIOTIC RESISTANCE, GENETIC DIVERSITY IN Klebsiella pneumoniae ISOLATED FROM FOODS OF ANIMAL ORIGIN AND ITS PUBLIC HEALTH SIGNIFICANCE
dc.these.typeM.V.Sc.
dc.titleDETECTION OF ANTIBIOTIC RESISTANCE, GENETIC DIVERSITY IN Klebsiella pneumoniae ISOLATED FROM FOODS OF ANIMAL ORIGIN AND ITS PUBLIC HEALTH SIGNIFICANCE
dc.typeThesis
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