COMPARATIVE PROTEOMIC ANALYSIS OF IMMATURE BUFFALO OOCYTES OF HIGH AND LOW DEVELOPMENTAL COMPETENCE
| dc.contributor.advisor | P. PALTA | |
| dc.contributor.author | RAGHVENDRA KUMAR | |
| dc.date.accessioned | 2023-11-17T15:41:23Z | |
| dc.date.available | 2023-11-17T15:41:23Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Selecting oocytes of high developmental competence is essential for the success of several reproductive technologies. The objectives of the present study were i) identification of proteins differentially expressed in immature oocytes of high and low developmental competence, selected by brilliant cresyl blue (BCB) staining, ii) to study the functional relevance of the differentially expressed proteins using bioinformatics tools and iii) validation of important differentially expressed proteins associated with the developmental competence of oocyte. Immature oocytes obtained from abattoir buffalo ovaries were classified into usable and unusable categories based on morphological criteria, and those of usable category were further classified into oocytes of high (BCB+) and low (BCB-) developmental competence after staining them with BCB. The oocytes of both the groups were denuded of their cumulus mass and lysed. Proteins were isolated and subjected to iTRAQ for proteome analysis. The data obtained was subjected to bioinformatics analysis using PANTHER, KEGG, DAVID, STRING, CYTOSCAPE and METSCAPE. A total of 692 proteins were found to be differentially expressed between BCB+ and BCB- oocytes. Following analysis focused on only three related species i.e., Bos taurus, Bubalus bubalis and Bos grunniens, 296 proteins were found to be differentially expressed between the two groups. Among these, 67 proteins were upregulated (FC≥1.2, FDR ≤ 1%) and 9 down-regulated (FC≤0.83, FDR ≤ 1%) in BCB+ compared to BCB- oocytes. The top ten up-regulated proteins were PFKL, MVP, GLRX, PGK1, TUBA1D, APMAP, LDHA, HMGN1, ENO1 and YWHAE whereas, the downregulated proteins included, A1BG, AHSG, APOC3, COX7A2, FETUB, GC, HBA, NDUFS6 and SERPINA1. Through bioinformatics analysis we found proteins from metabolic pathways, ribosomes, thermeogenesis, spliceosome, oxidative phosphorylation, glycolysis/ gluconeogenesis, endoplasmic reticulum protein processing (redox homeostasis) and from oocyte meiosis. The significantly differentially expressed proteins were involved in 121 pathways out of which mostly were metabolic pathways suggesting that the metabolic pathways may be playing an important role in determining the developmental competence of oocytes. The results indicated that the developmental competence of immature oocytes may be associated with regulation of translation including initiation, elongation and termination steps and that compromised carbohydrate metabolism may contribute to low developmental competence. MVP, LDHA, RPL23A, RPS11 and PDIA4 were found to be candidate biomarkers of developmental competence which need to be studied further to identify the best biomarker among them. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810201437 | |
| dc.language.iso | English | |
| dc.pages | 76 p. | |
| dc.publisher | ICAR-NDRI, KARNAL | |
| dc.sub | Animal Biotechnology | |
| dc.theme | ANIMAL BIOTECHNOLOGY | |
| dc.these.type | Ph.D | |
| dc.title | COMPARATIVE PROTEOMIC ANALYSIS OF IMMATURE BUFFALO OOCYTES OF HIGH AND LOW DEVELOPMENTAL COMPETENCE | |
| dc.type | Thesis |