Micropropagation studies in important cultivars of bamboo (Bambusa bambos & Dendrocalamus asper)

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dc.contributor.advisorChowdhury, V.K.
dc.contributor.authorRam Niwash
dc.date.accessioned2016-11-09T14:27:48Z
dc.date.available2016-11-09T14:27:48Z
dc.date.issued2011
dc.description.abstractThe present investigation has been undertaken to: (i) To develop efficient micropropagation protocol in two important cultivars of bamboo (Bambusa bambos & Dendrocalamus asper). (ii) Standardization of protocol and transplantation of regenerated plantlets of both cultivars to green house. Maximum survival percentage of explants (91.7%) in Bambusa bambos and 83.3% in Dendrocalamus asper was obtained when the nodal explants were sterilized with 0.2% bavistin (45 minutes), 0.2% streptocyclin (15 minutes) and 0.1% HgCi2 (6 minutes) in series. Bambusa bambos was observed to be more responsive to shoot proliferation and multiplication as compared to Dendrocalamus asper. Maximum per cent shoot proliferation was observed (88.9%) on MS basal medium supplemented with 2.0 mg/l BAP, 0.5 mg/l kinetin and 0.25 mg/l NAA in Bambusa bambos and 77.8% in Dendrocalamus asper and average number of shoot proliferation per explant was 2.5 in Bambusa bambos and 1.5 in Dendrocalamus asper. Maximum shoot multiplication response was observed in Bambusa bambos with an average of 8.4 shoot regenerated per explants and 5.4 in Dendrocalamus asper on MS basal medium supplemented with 2.0 mg/l BAP, 0.5 mg/l kinetin, 0.20 mg/l NAA and 30.0 mg/l adenine sulphate in both the cultivars. Maximum root induction frequency was obtained when half strength MS media supplemented with 2.0mg/l IBA used and 77.8% rooting after 7 days in Bambusa bambos and 66.7% rooting after 10 days in Dendrocalamus asper respectively. Survival rate of regenerated plantlets transferred to potting mixture containing sand + soil + vermicompost (1:1:1) was 83.3% in Bambusa bambos and 75.0% in Dendrocalamus asper.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/84713
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subMolecular Biology and Biotechnology
dc.subjectPlanting, Forest products, Biological phenomena, Vegetative propagation, Biological development, Auxins, Tissue culture, Iba, Icropropagation, Developmental stagesen_US
dc.these.typeM.Sc
dc.titleMicropropagation studies in important cultivars of bamboo (Bambusa bambos & Dendrocalamus asper)en_US
dc.typeThesisen_US
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