Micropropagation studies in important cultivars of bamboo (Bambusa bambos & Dendrocalamus asper)
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Date
2011
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Publisher
CCSHAU
Abstract
The present investigation has been undertaken to: (i) To develop efficient
micropropagation protocol in two important cultivars of bamboo (Bambusa bambos &
Dendrocalamus asper). (ii) Standardization of protocol and transplantation of regenerated
plantlets of both cultivars to green house. Maximum survival percentage of explants (91.7%) in
Bambusa bambos and 83.3% in Dendrocalamus asper was obtained when the nodal explants were
sterilized with 0.2% bavistin (45 minutes), 0.2% streptocyclin (15 minutes) and 0.1% HgCi2 (6
minutes) in series. Bambusa bambos was observed to be more responsive to shoot proliferation and
multiplication as compared to Dendrocalamus asper. Maximum per cent shoot proliferation was
observed (88.9%) on MS basal medium supplemented with 2.0 mg/l BAP, 0.5 mg/l kinetin and 0.25
mg/l NAA in Bambusa bambos and 77.8% in Dendrocalamus asper and average number of shoot
proliferation per explant was 2.5 in Bambusa bambos and 1.5 in Dendrocalamus asper. Maximum
shoot multiplication response was observed in Bambusa bambos with an average of 8.4 shoot
regenerated per explants and 5.4 in Dendrocalamus asper on MS basal medium supplemented with 2.0
mg/l BAP, 0.5 mg/l kinetin, 0.20 mg/l NAA and 30.0 mg/l adenine sulphate in both the cultivars.
Maximum root induction frequency was obtained when half strength MS media supplemented with
2.0mg/l IBA used and 77.8% rooting after 7 days in Bambusa bambos and 66.7% rooting after 10 days
in Dendrocalamus asper respectively. Survival rate of regenerated plantlets transferred to potting
mixture containing sand + soil + vermicompost (1:1:1) was 83.3% in Bambusa bambos and 75.0% in
Dendrocalamus asper.
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Keywords
Planting, Forest products, Biological phenomena, Vegetative propagation, Biological development, Auxins, Tissue culture, Iba, Icropropagation, Developmental stages