Rapid Detection of the Marek’s Disease Viral Genome in Chicken Feathers by Loop-Mediated Isothermal Amplification

Simple item page
dc.contributor.authorRaja, Angamuthu
dc.contributor.authorSubasty, Baskaran
dc.contributor.authorDhinakar Raj, Gopal, et al.
dc.contributor.authorTANUVAS
dc.date.accessioned2020-02-21T11:48:42Z
dc.date.available2020-02-21T11:48:42Z
dc.date.issued2011
dc.descriptionTNV_JCM_2011_961-965en_US
dc.description.abstractA loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek’s disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did the LAMP primers have binding sites for the common avian DNA viruses (reticuloendotheliosis virus, chicken anemia virus, subgroup J of the avian leukosis virus). Additionally, the assay could detect up to 10 copies of the MEQ gene in theMDviral genome, and it had 10 times higher sensitivity than the traditional PCR methods. The LAMP master mix was stable for 90 days at 20°C. Furthermore, the efficiency of LAMP for detection of serotype 1 MDV in clinical samples was comparable to those of PCR and viral isolation. The LAMP procedure is simple and does not rely on any special equipment. The detection of serotype 1 MDV by LAMP will be useful for detecting and controlling oncogenic Marek’s disease.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810143418
dc.keywordsMarek’s Disease Viral Genome, Chicken Feathers, Loop-Mediated Isothermal Amplificationen_US
dc.language.isoenen_US
dc.pages961-965en_US
dc.subjectVeterinary Scienceen_US
dc.titleRapid Detection of the Marek’s Disease Viral Genome in Chicken Feathers by Loop-Mediated Isothermal Amplificationen_US
dc.title.alternativeJournal of Clinical Microbiologyen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_JCM_2011_961-965.pdf
Size:
554.02 KB
Format:
Adobe Portable Document Format
Description:
TNV_JCM_2011_961-965
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Articles (1.Journal)