MOLECULAR CHARACTERISATION OF THE VARIABILITY IN CELLULAR LEVEL TOLERANCE IN RICE (Oryza sativa L.). AN ANALYSIS BASED ON METABOLOMICS APPROACH
| dc.contributor.advisor | SHESHSHAYEE | |
| dc.contributor.author | PUSHPA, D. | |
| dc.date.accessioned | 2018-03-01T05:11:25Z | |
| dc.date.available | 2018-03-01T05:11:25Z | |
| dc.date.issued | 2016-10-20 | |
| dc.description.abstract | Aerobic cultivation can save up to 60 per cent of irrigation water in comparison with lowland rice, concomitant yield loss of upto 40 per cent is often noticed in aerobic cultivation, which is caused primarily due to an increased spikelet sterility. Better root systems coupled with higher cellular level tolerance (CLT) can significantly improve spikelet fertility under semi-irrigated aerobic condition. Cellular level tolerance (CLT) associated with production of compatible solutes and metabolites that reorganize proteins and cellular structures leading to maintenance of leaf turgor, cellular redox balance and homeostasis. Comprehensive profiling of solutes and metabolites in rice genotypes with varied level of drought tolerance and spikelet fertility would pave way in understanding the biochemical mechanisms at the cellular level and to identify genes responsible for high CLT under drought conditions. Fingerprint of biochemical perturbations could also be a reliable method to phenotype CLT under stress. Metabolite-based genome-wide association study (mGWAS) has emerged as a powerful alternative forward genetics strategy. In this study, differentially accumulated metabolites among drought tolerant and susceptible rice genotypes under well watered and water stress were measured using high resolution LC-MS. Metabolites that accumulated with significantly higher abundance in drought tolerant rice genotype compared to susceptible genotype upon stress induction were identified as Tolerance Related Induced (TRI) metabolites. Such TRI metabolites mainly belonged to primary metabolites: sugars, amino acids and secondary metabolites such as phenylproponoids, flavonoids and alkaloids. Primary metabolites mainly involved in synthesis of stress responsive proteins and osmoprotection and secondary metabolites as antioxidants, Reactive Oxygen Species (ROS) scavengers, coenzymes, UV and excess radiation screen and also as regulatory molecules. mGEWAS analysis identified genomic regions (QTL’s) associated with CLT and regulatory genes governing raffinose and GABA biosynthesis under drought stress in tolerant genotype. | en_US |
| dc.identifier.other | Th-11536 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810041459 | |
| dc.language.iso | en | en_US |
| dc.pages | 213 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
| dc.sub | Crop Physiology | en_US |
| dc.subject | null | en_US |
| dc.theme | MOLECULAR CHARACTERISATION OF THE VARIABILITY | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | MOLECULAR CHARACTERISATION OF THE VARIABILITY IN CELLULAR LEVEL TOLERANCE IN RICE (Oryza sativa L.). AN ANALYSIS BASED ON METABOLOMICS APPROACH | en_US |
| dc.type | Thesis | en_US |