Analysis of DRO1 expression under water deficit stress and its relationship with root angle phenotype in Indian rice accessions
| dc.contributor.advisor | S. V. Amitha, C. R. Mithra | |
| dc.contributor.author | BABLEE KUMARI SINGH | |
| dc.date.accessioned | 2018-08-13T09:01:30Z | |
| dc.date.available | 2018-08-13T09:01:30Z | |
| dc.date.issued | 2017 | |
| dc.description | t-9658 | en_US |
| dc.description.abstract | Deeper Rooting 1 (DRO1) gene identified from a major QTL on chromosome 9 is associated with increasing the root growth angle (RGA) in rice. This gene is a major player in providing wider RGA and facilitates survival under water deficit stress conditions. To understand the allelic status and expression of DRO1 and its association with RGA phenotype in rice the present research work was undertaken. The study material comprising of 24 major Indian upland and lowland genotypeswere grown in well-watered control (WC) and water-deficit stress (WS) conditions. Root length increased under WS across the accessions wherein the genotypes Azucena and Satti showed almost 40% increase.For root length, ANOVA revealed that the genotype differences were not significant but treatment differences were. In case of root angle, however, the means were statistically non-significant between the treatments while genotype differences did exist based on which the study material could be grouped into two classes (9 wide angle and 15 narrow angle genotypes). Expression analysis revealed that the gene was upregulated in all genotypes by 1- 4.5 fold and verified the grouping in seven of the nine wider angle genotypes. DRO1 gene (3058bp) was amplified using four pairs of overlapping primers and sequenced. In the 756 bp long CDS of DRO1, a total of 52 SNPs but no InDels were identified across the 24 genotypes. The SNP position 375 was triallelic and transversions were almost 2.5 folds more common than transitions. Among the genotypes, donor of DTY 3.1(1 SNP) showed the least number of changes, whereas DTY 9.1(19 SNPs) showed the most. In the 251 amino acid long protein, substitutions were found in 19 positions, wherein position 92 was the most frequent. Based on the allele mining, all the 24 genotypes can be grouped into 16 haplotypes covering the variation from functional allele (Satti, IR36 and DTY 3.1) to truncated non-functional alleles in PMK2, IR64, IR20 and Swarna. The information obtained in the present investigation regarding the allelic and expression status of the DRO1 gene will be very useful for the breeders in their crop improvement programme on drought tolerance. Key words: Rice, root angle, DRO1, allele mining, expression profiling | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810064222 | |
| dc.keywords | Rice, root angle, DRO1, allele mining, expression profiling | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | DIVISION OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI | en_US |
| dc.sub | Biotechnology and Molecular Biology | |
| dc.subject | null | en_US |
| dc.theme | Analysis of DRO1 expression under water deficit stress and its relationship with root angle phenotype in Indian rice accessions | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Analysis of DRO1 expression under water deficit stress and its relationship with root angle phenotype in Indian rice accessions | en_US |
| dc.type | Thesis | en_US |